ORIGINAL ARTICLE  EPIDEMIOLOGY AND CLINICAL MEDICINE 

The Journal of Sports Medicine and Physical Fitness 2020 March;60(3):435-41

DOI: 10.23736/S0022-4707.19.10113-2

Copyright © 2019 EDIZIONI MINERVA MEDICA

lingua: Inglese

Exercise-induced BDNF production by mononuclear cells of sedentary and physically active obese men

Viviane R. ELSNER ¹, Gilson P. DORNELES ²✉, Maeli A. SANTOS ², Igor M. da SILVA ², Pedro ROMÃO ², Alessandra PERES ²

¹ Methodist University - IPA, Porto Alegre, Brazil; ² Cellular and Molecular Immunology Lab, Department of Health Basic Sciences, Federal University of Health Sciences of Porto Alegre, Porto Alegre, Brazil

BACKGROUND: Obesity and low physical activity changes the redox state and neurotrophin secretion by leukocytes. However, the role of exercise on brain derived neurotrophic factor (BDNF) production and oxidative stress markers of peripheral blood mononuclear cells (PBMC) remains unknown. This study aimed to verify the impact of acute maximal exercise on oxidative stress markers and the BDNF production by stimulated PBMC from sedentary and physically active obese men.
METHODS: PBMC from twelve sedentary obese (SED group) and twelve regular exercisers (EXE group) obese men were collected before, immediately and 1-h after maximal exercise. PBMC were stimulated with lipopolysaccharide (LPS) to evaluate the BDNF and nitrite production, lipid peroxidation, and antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD) activities.
RESULTS: PBMC from EXE group presented higher BDNF production (P=0.03) and lower TBARS levels than SED group at baseline. Maximal exercise increased BDNF and nitrite production, and lipid peroxidation immediately and 1-h after the bout in both groups. The EXE group presented higher superoxide dismutase activity immediately after bout and higher catalase activity 1-h after bout in PBMC. On the other hand, PBMC from SED group had lower superoxide dismutase activity immediately after exercise. Furthermore, PBMC from EXE group presented higher BDNF production and SOD activity and lower TBARS concentrations than SED group immediately after maximal exercise.
CONCLUSIONS: Maximal exercise changes the redox state and enhances BDNF production by LPS-stimulated PBMC in obese individuals.

KEY WORDS: Nerve growth factors; Oxidative stress; Leukocytes; Exercise; Cardiorespiratory fitness; Obesity