Panminerva Medica 2020 Apr 28

DOI: 10.23736/S0031-0808.20.03920-8

Copyright © 2020 EDIZIONI MINERVA MEDICA

lingua: Inglese

MiR-99b regulates cerebral ischemia neuronal injury through targeting IGF1R

QI DENGBIN ¹, Wang WEI ², Zhang YING ³, Zhang TAO ⁴ ✉

¹ Department of Neurology, Affiliated Hospital of Jining Medical University, YanZhou Branch, Jining, P.R. China; ² Disinfection Supply Center, Qingdao Municipal Hospital, Qingdao, P.R. China; ³ Department of Internal Medicine, Binzhou People's Hospital, Binzhou, P.R. China; ⁴ Department of Cardiovascular Surgery, Provincial Hospital Affiliated to Shandong University, Jinan, P.R. China

BACKGROUND: Recently, microRNA-99b (miR-99b) shows diverse functions in different human disease. However, further studies about the potential effect of miR-99b in cerebral ischemia injury still need to be done.
METHODS: The expressions of miR-99b and IGF1R were detected via RT-qPCR assay. Western blot assay was applied to measure the protein expression of Caspase-3, Bax and Bcl-2. MTT assay was used to observe cell viability of SH-SY5Y cells. The association of miR-99b and IGF1R was testified by dual luciferase assay. And human SH-SY5Y cells were treated with the oxygen-glucose deprivation / reperfusion (OGD/R) to mimic CIR injury.
RESULTS: The expression of miR-99b was increased in the OGD/R model. And upregulation of miR-99b promoted cell viability and inhibited apoptosis induced by OGD/R. Moreover, IGF1R was confirmed as a direct target gene of miR-99b. The expression of IGF1R was obviously decreased under OGD/R conditions.
CONCLUSIONS: MiR-99b promoted the viability and suppressed apoptosis of SH-SY5Y cells under OGD/R conditions through targeting IGF1R.

KEY WORDS: miR-99; Cerebral ischemia injury; IGF1R; Apoptosis