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Panminerva Medica 2020 Apr 28
DOI: 10.23736/S0031-0808.20.03920-8
Copyright © 2020 EDIZIONI MINERVA MEDICA
lingua: Inglese
MiR-99b regulates cerebral ischemia neuronal injury through targeting IGF1R
QI DENGBIN 1, Wang WEI 2, Zhang YING 3, Zhang TAO 4 ✉
1 Department of Neurology, Affiliated Hospital of Jining Medical University, YanZhou Branch, Jining, P.R. China; 2 Disinfection Supply Center, Qingdao Municipal Hospital, Qingdao, P.R. China; 3 Department of Internal Medicine, Binzhou People's Hospital, Binzhou, P.R. China; 4 Department of Cardiovascular Surgery, Provincial Hospital Affiliated to Shandong University, Jinan, P.R. China
BACKGROUND: Recently, microRNA-99b (miR-99b) shows diverse functions in different human disease. However, further studies about the potential effect of miR-99b in cerebral ischemia injury still need to be done.
METHODS: The expressions of miR-99b and IGF1R were detected via RT-qPCR assay. Western blot assay was applied to measure the protein expression of Caspase-3, Bax and Bcl-2. MTT assay was used to observe cell viability of SH-SY5Y cells. The association of miR-99b and IGF1R was testified by dual luciferase assay. And human SH-SY5Y cells were treated with the oxygen-glucose deprivation / reperfusion (OGD/R) to mimic CIR injury.
RESULTS: The expression of miR-99b was increased in the OGD/R model. And upregulation of miR-99b promoted cell viability and inhibited apoptosis induced by OGD/R. Moreover, IGF1R was confirmed as a direct target gene of miR-99b. The expression of IGF1R was obviously decreased under OGD/R conditions.
CONCLUSIONS: MiR-99b promoted the viability and suppressed apoptosis of SH-SY5Y cells under OGD/R conditions through targeting IGF1R.
KEY WORDS: miR-99; Cerebral ischemia injury; IGF1R; Apoptosis