ORIGINAL ARTICLE   

Panminerva Medica 2021 September;63(3):336-42

DOI: 10.23736/S0031-0808.20.03939-7

Copyright © 2020 EDIZIONI MINERVA MEDICA

lingua: Inglese

MicroRNA-1271-5p inhibits the tumorigenesis of ovarian cancer through targeting E2F5 and negatively regulates the mTOR signaling pathway

Qin LI, Junyu SHI, Xiaoli XU ✉

Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Soochow University (The First People’s Hospital of Changzhou), Changzhou, China

BACKGROUND: MicroRNA-1271-5p (miR-1271-5p) has been reported to participate in the progression of many human cancers. However, the role of miR-1271-5p still remains unclear in ovarian cancer (OC). Therefore, we explored the effect of miR-1271-5p on the development of OC in present study.
METHODS: We measured the miR-1271-5p expression via the qRT-PCR assay. Then the function of miR-1271-5p was analyzed through MTT and Transwell assays. The relationship among miR-1271-5p and E2F5 was verified by dual luciferase assay. The protein expression levels were examined through western blot.
RESULTS: MiR-1271-5p was downregulated in OC tissues which predicted poor prognosis of OC patients. Moreover, E2F5 was a direct target of miR-1271-5p in OC. And miR-1271-5p suppressed cell proliferation, migration and invasion in OC through targeting E2F5. Furthermore, E2F5 was upregulated in OC tissues which predicted poor prognosis of OC patients. Besides that, miR-1271-5p suppressed EMT and mTOR pathway in OC.
CONCLUSIONS: MiR-1271-5p inhibited the tumorigenesis of OC through targeting E2F5 and negatively regulated the mTOR signaling pathway.

KEY WORDS: Ovarian neoplasms; Blotting, western; Proteins