USE OF RADIOLABELLED PEPTIDES FOR SCINTIGRAPHY IN NON-ONCOLOGIC DISEASES 

The Quarterly Journal of Nuclear Medicine 2003 December;47(4):349-61

Copyright © 2009 EDIZIONI MINERVA MEDICA

lingua: Inglese

The role of labeled Annexin A5 in imaging of programmed cell death. From animal to clinical imaging

Kietselaer B. L. J. H. ¹, Hofstra L. ¹, Dumont E. A. W. ¹, Reutelingsperger C. P. M. ², Heidendal G. A. K. ³

¹ Department of Cardiology University Hospital of Maastricht, Maastricht, The Netherlands 2 Department of Biochemistry University of Maastricht, Maastricht, The Netherlands 3 Department of Nuclear Medicine University Hospital of Maastricht, Maastricht, The Netherlands

Programmed ­cell ­death ­plays a crit­i­cal ­role in embryo­lo­gy, homeo­sta­sis and dis­ease. However, ­until recent­ly no non-inva­sive imag­ing modal­ity has ­been ­able to vis­u­al­ize ­this pro­cess direct­ly. Annexin A5 ­binds to ­cells under­go­ing pro­grammed ­cell ­death. When label­ing ­this pro­tein, Annexin A5 ­becomes a ­tool for the detec­tion of pro­grammed ­cell ­death in ­vitro and in ­vivo. Labeled Annexin A5 has ena­bled our ­group and oth­ers to ­detect pro­grammed ­cell ­death non-inva­sive­ly in ani­mals and ­patients. This ­review ­will high­light the devel­op­ment of ­this imag­ing modal­ity in cel­lu­lar and ani­mal mod­els. Furthermore, we ­will dis­cuss Annexin A5 imag­ing in ­human dis­ease. We ­will ­focus on the clin­i­cal appli­ca­tions and ­their rel­e­vance, lim­i­ta­tions and ­future per­spec­tives of non-inva­sive imag­ing of pro­grammed ­cell ­death ­using ­labeled Annexin A5.