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Minerva Stomatologica 2019 February;68(1):36-41

DOI: 10.23736/S0026-4970.18.04172-9

Copyright © 2018 EDIZIONI MINERVA MEDICA

lingua: Inglese

Interaction of intracanal medicaments with apical papilla stem cells: quantitative cytotoxicity assessment by methyl thiazolyl tetrazolium, trypan blue and lactate dehydrogenase

Eshaghali SABERI 1, Narges FARHAD-MOLLASHAHI 1 , Mersad SABERI 2

1 Department of Endodontics, Oral and Dental Diseases Research Center, Faculty of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran; 2 Private practitioner, Zahedan, Iran



BACKGROUND: Chemical residues often have cytotoxic effects on the stem cells. This study aimed to assess the cytotoxic effects of intracanal medicaments on stem cells of the apical papilla (SCAPs) using methyl thiazolyl tetrazolium (MTT), trypan blue exclusion (TBE) and lactate dehydrogenase release (LDHR) assays.
METHODS: SCAPs were cultured and exposed to 0.125, 0.25, 1, 5 and 10 mg/mL concentrations of modified triple antibiotic paste (mTAP)/distilled water (DW), mTAP/chlorhexidine (CHX), calcium hydroxide(Ca(OH)2)/CHX and Ca(OH)2/DW. Cell viability was quantitatively analyzed using the MTT, LDHR and TBE assays. Data were analyzed using one-way ANOVA and Tukey’s test.
RESULTS: All three assessment methods yielded the same results. Ca(OH)2/ DW resulted in the highest and mTAP/CHX resulted in the lowest cell viability. In contrast to Ca(OH)2, mTAP decreased cell viability in a dose-dependent manner. Also, addition of CHX to mTAP and Ca(OH)2 increased their cytotoxicity.
CONCLUSIONS: In contrast to the proliferative effects of Ca(OH)2, even low concentrations of mTAP have cytotoxic effects on SCAPs.


KEY WORDS: Anti-bacterial agents - Calcium hydroxide - Stem cells - L-lactate dehydrogenase - Trypan blue

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