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Minerva Pediatrica 2020 May 15

DOI: 10.23736/S0026-4946.20.05765-5


lingua: Inglese

Anti-inflammatory activity of a fixed combination of probiotics and herbal extract in an in vitro model of intestinal inflammation by stimulating Caco-2 cells with LPS- conditioned THP-1 cells medium

Vittoria BORGONETTI 1, Veronica COCETTA 1, Marco BIAGI 2, Ilaria CARNEVALI 3, Paolo GOVERNA 4 , Monica MONTOPOLI 1

1 Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Padua, Italy; 2 Department of Physical Sciences, Earth and Environment, University of Siena, Siena, Italy; 3 Clinical Research Department Schwabe Pharma Italia, Egna, Bolzano, Italy; 4 Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Siena, Italy


BACKGROUND: Inflammatory bowel disease (IBD) is an inflammatory condition of the gastrointestinal tract, characterized by chronic and relapsing immune system activation, often diagnosed in adolescence, with a rising incidence in pediatric populations. IBD results from altered interactions between gut microbes and the intestinal immune system which induce an aberrant immune response, thus anti-inflammatory or immunosuppressive therapies are generally used. Recent interest has been given to the identification of integrative and complementary approaches that could be able to restore and preserve the intestinal barrier function.
METHODS: In this work, we tested the effect of a fixed combination of probiotics and herbal extract (ColikindGocce®, CKG) in an in vitro model of intestinal inflammation. CaCo-2cells stimulated with LPS-conditioned monocytes culture medium was used as a paradigm of intestinal inflammation. The possible effect of CKG in maintaining the homeostasis of the intestinal epithelial barrier was investigated by measurement of the trans- epithelial electrical resistance, the paracellular permeability, and the release of inflammatory cytokines (TNF-α, IL8, and IL10).
RESULTS: Results obtained in this work demonstrated that CKG is able to prevent the impairment of intestinal barrier function induced by inflammation, ameliorating the transepithelial electrical resistance and the paracellular permeability of the Caco-2 monolayer; moreover, CKG is able to counteract the increased release of TNF-a and IL-8 induced by inflammatory stimulus, thus reducing the intestinal inflammation.
CONCLUSIONS: This work underlines the protective effect of CKG on intestinal barrier, reducing the damages induced by inflammatory stimulus. This suggests CKG as an interesting product in the management of intestinal inflammatory conditions.

KEY WORDS: Inflammatory bowel diseases; Caco-2 cells; Lactobacillus reuteri; Lactobacillus acidofilus; Trans-epithelial electrical resistance (TEER); THP-1 cells; Cytokines; Anti- inflammatory activity

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