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MINERVA PEDIATRICA

Rivista di Pediatria, Neonatologia, Medicina dell’Adolescenza
e Neuropsichiatria Infantile


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Minerva Pediatrica 2017 Sep 15

DOI: 10.23736/S0026-4946.17.04921-0

Copyright © 2017 EDIZIONI MINERVA MEDICA

lingua: Inglese

Evidence of correlation between TGFBR2 gene expression mediated by NF-kB signaling pathways and Kawasaki disease in children

Qinling GAO 1, Shuhua YUAN 2, Dawei YUAN 3

1 The Second Department of Pediatric Internal Medicine, Linyi People’s Hospital, Linyi, P.R. China; 2 Department of Neonatology, Linyi People’s Hospital, Linyi, P.R. China; 3 Department of Pediatric Internal Medicine, Linyi Women and Children Hospital, Linyi, P.R. China


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BACKGROUND: We explored the correlation between the TGFBR2 gene that is mediated by NF-kb signaling pathways and the pathogenesis of Kawasaki disease in children.
METHODS: In this study, 43 children with Kawasaki disease from April 2014 to January 2016 at our hospital were selected as the observation group, and 42 healthy children were selected as the control group. The mRNA expression levels of NF-kb gene and TGFBR2 gene in different groups were detected using fluorescence quantitative PCR. The protein expression levels of the NF-kb and TGFBR2 were detected using enzyme-linked immunosorbent assay (ELISA) in different groups. The expression levels of NF-kb and TGFBR2 in the observation group and the control group were detected using immunohistochemistry.
RESULTS: Compared to the control group, the mRNA expression levels of NF-kb and TGFBR2 were 12.3 times and 27.5 times as high as those in the control group respectively and there were significant differences between the two groups (p<0.05). ELISA results showed that there were significant differences between the protein expression levels of NF-kb and TGFBR2 in the control group (0.87±0.12, 1.25±0.18) ug/l and those in the observation group (3.27±0.17, 8.16±0.22) ug/l (p<0.05). Western-blotting results showed that the expression levels of the NF-kB and TGFBR2 in children with Kawasaki disease were significantly higher than those in healthy subjects (p<0.05). Immunohistochemistry results showed that the positive cell rate of TGFBR2 (89.7%) was significantly higher in children with Kawasaki disease than that in healthy children (4.5%); there was significant difference between the two groups (p<0.05).
CONCLUSIONS: The TGFBR2 may be involved in the pathogenesis of Kawasaki disease in children through NF-kB signaling pathways.


KEY WORDS: Nf-kb - Signaling pathways - TGFBR2 - Kawasaki disease in children - Correlation

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Publication History

Article first published online: September 15, 2017
Manuscript accepted: July 20, 2017
Manuscript revised: May 25, 2017
Manuscript received: February 8, 2017

Per citare questo articolo

Gao Q, Yuan S, Yuan D. Evidence of correlation between TGFBR2 gene expression mediated by NF-kB signaling pathways and Kawasaki disease in children. Minerva Pediatr 2017 Sep 15. DOI: 10.23736/S0026-4946.17.04921-0

Corresponding author e-mail

huangchoufeng03@163.com