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Minerva Medica 2019 February;110(1):18-26

DOI: 10.23736/S0026-4806.18.05865-2

Copyright © 2018 EDIZIONI MINERVA MEDICA

lingua: Inglese

Blood test strategy of blood donors, ALT and HBsAg HCV-Ab correlation study

Larang ZHENG 1, Xiaoyan LI 2

1 Department of Quality Management, Baoji Central Blood Station of Shaanxi Province, Baoji City, China; 2 Department of Blood Transfusion, Baoji Central Hospital of Shaanxi Province, Baoji City, China



BACKGROUND: The relationship between ALT and HBsAg and anti-HCV was explored. The effects of different detection methods on blood test results of blood donors were analyzed, and the best blood test mode for blood donors was proposed.
METHODS: Rate method was used to detect ALT, and ELISA was used to detect HBsAg, anti-HCV, anti-TP, and anti-HIV. Negative samples were detected by ELISA for NAT detection (PCR). HBV-NAT-negative specimens and HBV-NAT-positive specimens were detected by electrochemiluminescence for HBsAg, HBs-Ab, HBeAg, HBe-Ab, HBc-Ab (two-and-a-half). The ELISA reagents of different reagent manufacturers were evaluated by serum test disc and quality control method.
RESULTS: The total ALT failure rate of blood donors was 2.48%, of which the single ALT failure rate was 2.45%, accounting for 98.81% (5957/6029). The positive rate of HBsAg was 0.36%, the anti-HCV positive rate was 0.40%, the anti-TP positive rate was 0.46%, and the anti-HIV positive rate was 0.09%. ELISA-negative specimens were detected by NAT, HBV nucleic acid-positive 164 persons, accounting for 0.09%, no HCV, HIV-positive. HBV-NAT positive specimens were detected by nucleic acid quantitative test; the positive rate of HBV-DNA was 56.67%. HCV-positive blood donors were followed up for 24 weeks and re-examined; negative nucleic acid cases accounted for 98.92%. The detection results of serum test disc and quality control method were not the same.
CONCLUSIONS: There was no correlation between ALT positive and HBV and HCV in blood donors. HIV detection are better detection modes by two-pass enzyme elimination and one-time NAT detection (PCR), which can effectively reduce transfusion infection caused by occult infection of HBV and improve blood safety. HBsAg, HCV-Ab positive blood donors after ELISA and nucleic acid test negative after 24 weeks (maximum window period) are recommended to return to the blood donation team to participate in blood donation. The two ELISA reagents were tested by different manufacturers, which were complementary and improved the detection accuracy.


KEY WORDS: Blood donors - Hematologic test - Methods

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