ORIGINAL ARTICLE   

Minerva Medica 2018 April;109(2):95-102

DOI: 10.23736/S0026-4806.17.05349-6

Copyright © 2017 EDIZIONI MINERVA MEDICA

lingua: Inglese

Polyphenol epigallocatechin-3-gallate inhibits hypoxia/reoxygenation-induced H9C2 cell apoptosis

Wenlong WANG ¹, Xiaohui HUANG ², Deliang SHEN ², Zonghua MING ³, Mei ZHENG ⁴, Jinying ZHANG ² ✉

¹ Emergency Department, Beijing Shijitan Hospital, Beijing, China; ² Department of Cardiology, First Affiliated Hospital, Zhengzhou University, Zhengzhou, China; ³ Health Management Center, People’s Hospital of Zhangqiu, Jinan, China; ⁴ Department of Medicine, People’s Hospital of Zhangqiu, Jinan, China

BACKGROUND: We aimed to observe the protective effect of EGCG on hypoxia/reoxygenation injury of H9C2 myocardial cells and to study the inhibition mechanism of EGCG on hypoxia/reoxygenation injury of H9C2 myocardial cells.
METHODS: H9C2 cells were used as the objects of study and hypoxia/reoxygenation cells were pretreated with EGCG in different concentrations. MTT, Hoechst 33258 and LDH were used to detect the viability and apoptosis of H9C2 cells. The protection mechanism of polyphenol epigallocatechin-3-gallate was studied via western blotting and mitochondrial membrane potential detection.
RESULTS: Polyphenol epigallocatechin-3-gallate (10 μM) reduced the proportion of H9C2 cell apoptosis after hypoxia/reoxygenation (4/20 h), stabilized the mitochondrial membrane potential and decreased the expressions of mitochondrial damage-related proteins, thus protecting the mitochondrial function.
CONCLUSIONS: Polyphenol epigallocatechin-3-gallate alleviates the hypoxia-/reoxygenation-induced H9C2 cell apoptosis, which may play the myocardial protective effect through inhibiting the mitochondrial Caspase pathway.

KEY WORDS: Polyphenols - Epigallocatechin gallate - Hypoxia - Mitochondrial membrane potential - Myocardium