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Minerva Ginecologica 2020 May 13

DOI: 10.23736/S0026-4784.20.04566-9

Copyright © 2020 EDIZIONI MINERVA MEDICA

lingua: Inglese

Vaginal Lactobacillus species and inflammatory biomarkers in pregnancy

Kushal GANDHI 1, Paula GUTIERREZ 1, 2, John GARZA 1, 2, Taylor J. GRAY-WLAZLO 1, Rebecca J. MEISER 1, Samuel DAVID 3, Maira CARRILLO 4, Madhusudhanan NARASIMHAN 5, Michael GALLOWAY 1, Gary VENTOLINI 1

1 School of Medicine, Texas Tech University Health Sciences Center Permian Basin, Odessa, TX, USA; 2 The University of Texas of the Permian Basin, Odessa, TX, USA; 3 Shepherd University, Shepherdstown, WV, USA; 4 Odessa College, Odessa, TX, USA; 5 Department of Pharmacology and Neuroscience, Texas Tech University Health Sciences Center, Lubbock, TX, USA


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OBJECTIVE: To identify vaginal Lactobacillus spp. and quantify vaginal inflammatory cytokines in primigravida vs multigravida women and pregnant vs non-pregnant women.
STUDY DESIGN: Vaginal swabs were obtained from four groups of patients. A real-time PCR was carried out to identify the Lactobacillus spp. Multiplex immunoassays were performed to quantify a total of 27 cytokines using the Bio-Plex MAGPIX multiplex reader and MesoQuick Plex SQ 120. Inferential statistics using hypothesis tests were applied to detect differences in cytokine levels.
RESULTS: Significant differences in cytokines and chemokines exist among the four populations of women studied. IP-10 is significantly higher in multigravida women as compared to primigravida women. IFN-γ, MCP-1, MIP-1β, IL-2 and IL-10 are significantly higher in non-pregnant women compared to pregnant women. L. iners was the most abundant species in multigravida, pregnant and non-pregnant patients, while L. crispatus was the most abundant species in primigravida patients. Significant differences in the levels of MIP-1β, TNF-α, PDGF-BB, VEGF-A, IL-12, and IL-10 exist between women identified with Lactobacillus species and women not identified with Lactobacillus species.
CONCLUSIONS: There were significant differences regarding cytokines, chemokines, and Lactobacillus spp. among four groups of studied patients. With these results, we increase our understanding of the role that vaginal cytokines and Lactobacillus species have during pregnancy, with the goal that this novel research will be useful for examining vaginal biomarkers in obstetrical conditions.


KEY WORDS: Cytokine; Pregnancy; Lactobacillus species; Microbiome

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