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Minerva Endocrinologica 2020 Nov 19

DOI: 10.23736/S0391-1977.20.03278-2

Copyright © 2020 EDIZIONI MINERVA MEDICA

lingua: Inglese

Downregulation of plasma microRNA-29c-3p expression may be a new risk factor for diabetic retinopathy

Bora TORUS 1, Hakan KORKMAZ 2 , Kuyaş H. OZTURK 3, Fevziye B. ŞİRİN 4, Mehmet ARGUN 5, Sonmez ŞEVİK 4, Levent TÖK 5

1 Department of Internal Medicine, Faculty of Medicine, Suleyman Demirel University, Cunur, Isparta, Turkey; 2 Division of Endocrinology, Department of Internal Medicine, Faculty of Medicine, Suleyman Demirel University, Cunur, Isparta, Turkey; 3 Department of Medical Genetics, Faculty of Medicine, Suleyman Demirel University, Cunur, Isparta, Turkey; 4 Department of Biochemistry, Faculty of Medicine, Suleyman Demirel University, Cunur, Isparta, Turkey; 5 Department of Ophthalmology, Faculty of Medicine, Suleyman Demirel University, Cunur, Isparta, Turkey


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BACKGROUND: Circulation miRNAs have emerged as new biomarkers for identifying and monitoring the microvascular complications of diabetes. The aim of this study is to evaluate the levels of five candidate miRNAs (miR-29c-3p, miR-18a, miR-31, miR-181 and miR-20a) in patients with diabetic retinopathy (DR) and their relationship with disease severity.
METHODS: The study included 31 diabetes patients without DR (NDR group), 68 patients with DR (DR group) and 30 healthy controls (HC group). Twenty-five of patients with DR were proliferative DR (PDR group) and 43 were non-proliferative DR (NPDR group) patients. Metabolic parameters and serum vascular endothelial growth factor (VEGF) levels of all participants were measured. Circulating miRNAs levels were determined by quantitative realtime PCR. Fundus examinations of all patients were performed by a single ophthalmologist.
RESULTS: VEGF levels were significantly higher in the NDR and DR groups compared to HC group (p=0.011 and p=0.014, respectively). Plasma miR-29c-3p was downregulated in diabetic patients with retinopathy and without retinopathy. This downregulation was more prominent in diabetic patients without retinopathy compared to those with retinopathy (p=0.016). There was no significant difference in plasma levels of miR-18a, miR-20a, miR-18a and miR-31 between diabetic subjects with and without retinopathy (p>0.05). There was no correlation between DR severity and the levels of miRNAs (p>0.05). In multivariate logistic regression analysis, it was found that changes in plasma miR-29c-3p expression of diabetic patients increased DR risk independent of other risk factors.
CONCLUSIONS: Plasma miR-29c-3p expression is downregulated in diabetic patients with and without retinopathy, and changes in this miRNA are an independent risk factor for the development of DR.


KEY WORDS: Diabetic retinopathy; miR-29c-3p; Retinal angiogenesis; VEGF

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