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Minerva Dental and Oral Science 2021 Oct 05

DOI: 10.23736/S2724-6329.21.04538-1

Copyright © 2021 EDIZIONI MINERVA MEDICA

lingua: Inglese

Multiple approaches to oral epithelial dysplasia degree analyses: a pilot study

Carla M. RAMÍREZ-MARTÍNEZ 1, David A. TREJO-REMIGIO 2, Emiliano JURADO-CASTAÑEDA 3, Alejandro ALONSO-MOCTEZUMA 4, Diana RIVERA-REZA 1, Elba R. LEYVA-HUERTA 1, Javier PORTILLA-ROBERTSON 5, Luis F. JACINTO-ALEMÁN 1

1 Postgraduate and Research Division, Oral Medicine and Pathology Department, Dentistry School, National Autonomous University of Mexico, Mexico City, Mexico; 2 Cell Culture and Immunohistochemistry Laboratory, Oral Medicine and Pathology Department, Postgraduate and Research Division, Dentistry School, National Autonomous University of Mexico, Mexico City, Mexico; 3 Oral Medicine Clinic, Oral Medicine and Pathology Department, Postgraduate and Research Division, Dentistry School, National Autonomous University of Mexico, Mexico City, Mexico; 4 Oral and Maxillofacial Surgery Specialty Postgraduate and Research Division, Dentistry School, National Autonomous University of Mexico, Mexico City, Mexico; 5 Coordination of Oral Medicine and Pathology Department, Postgraduate and Research Division, Dentistry School, National Autonomous University of Mexico, Mexico City, Mexico


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BACKGROUND: Oral epithelial dysplasia (OED) is the presence of cells of an abnormal type within a tissue, which may signify a stage preceding the development of cancer. Our aim was to determine the interrelation between the expression of multiple molecular markers and the histological features of oral dysplasia.
METHODS: Fifteen samples of OED (five for each severity degree) were analyzed through software assisted image cytometry nuclear morphology. p53 (wildtype and mutated form), Bax and Bcl2 expression was immunohistochemically determined, and the gene expression of MMP1, MMP2,MMP9 and hTERT was determined by RT-PCR. The mean, standard deviation, ANOVA and Fisher´s exact test (p<0.05) were performed.
RESULTS: Our analysis indicated congruence between the software-assisted measurement of nuclear morphology and severity degree. Only five samples were positive to p53-mutated form; and Bax was more expressed than Bcl-2. hTERT expression was significantly expressed in relation to severity, and MMP1 was predominantly expressed, followed by MMP9 and MMP2.
CONCLUSIONS: Our results reinforce that software-assisted measurement is an alternative to severity degree determination. MMP1 is an important marker for severity dysplasia degree, however the predominant expression of Bax over Bcl-2 suggests that this pro-apoptotic state could be used to minorize the progression, perhaps, as a future therapeutic target.


KEY WORDS: Nuclear morphometric; p53; Bax; MMP1; hTERT

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