ORIGINAL ARTICLE   

Minerva Dental and Oral Science 2022 December;71(6):346-52

DOI: 10.23736/S2724-6329.22.04425-4

Copyright © 2022 EDIZIONI MINERVA MEDICA

lingua: Inglese

Evaluation of gingival crevicular fluid interleukin-35 as a marker for identification of periodontal disease activity

Kaustubh S. THAKARE ¹ ✉, Priti A. CHARDE ², Manohar L. BHONGADE ², Priyanka P. JAISWAL ², Pavan S. BAJAJ ²

¹ Department of Periodontology and Implantology, VYWS Dental College, Amravati, Maharashtra, India; ² Department of Periodontology and Implantology, Sharad Pawar Dental College and Hospital, Wardha, Maharashtra, India

BACKGROUND: This study was carried out to evaluate and compare the levels of IL-35 in gingival crevicular fluid (GCF) in periodontally healthy subjects, patients with gingivitis and chronic periodontitis and to assess IL-35 as a marker for identification of periodontal disease activity.
METHODS: GCF samples were obtained from periodontally healthy subjects (N.=15), gingivitis patients (N.=15) and patients with chronic periodontitis (N.=15). Clinical measurements like probing pocket depth, clinical attachment loss, bleeding on probing, Papillary Bleeding Index, and Modified Plaque Index were recorded. Enzyme-linked immunosorbent assay was used for the determination of GCF IL-35 levels in samples.
RESULTS: The IL-35 levels were significantly higher in the healthy subjects as compared to gingivitis and chronic periodontitis group. There was variation in GCF IL-35 levels in healthy sites in each group and gingivitis sites in gingivitis and chronic periodontitis patients.
CONCLUSIONS: The levels of IL-35 were observed to decrease with increase in the inflammatory status, so it might play a role in suppressing gingival inflammation and maintaining periodontal health.

KEY WORDS: Enzyme-linked immunosorbent assay; Gingival crevicular fluid; Gingivitis; Interleukin-35; Periodontitis