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Minerva Dental and Oral Science 2022 April;71(2):59-65

DOI: 10.23736/S2724-6329.21.04538-1

Copyright © 2021 EDIZIONI MINERVA MEDICA

lingua: Inglese

Multiple approaches to oral epithelial dysplasia degree analyses: a pilot study

Carla M. RAMÍREZ-MARTÍNEZ 1, David A. TREJO-REMIGIO 2, Emiliano JURADO-CASTAÑEDA 3, Alejandro ALONSO-MOCTEZUMA 4, Diana I. RIVERA-REZA 1, Elba R. LEYVA-HUERTA 1, Javier PORTILLA-ROBERTSON 1, Luis F. JACINTO-ALEMÁN 1

1 School of Dentistry, Postgraduate and Research Division, Department of Oral Medicine and Pathology, National Autonomous University of Mexico, Mexico City, Mexico; 2 School of Dentistry, Postgraduate and Research Division, Department of Oral Medicine and Pathology, Laboratory of Cell Culture and Immunohistochemistry, National Autonomous University of Mexico, Mexico City, Mexico; 3 School of Dentistry, Postgraduate and Research Division, Department of Oral Medicine and Pathology, Clinic of Oral Medicine, National Autonomous University of Mexico, Mexico City, Mexico; 4 School of Dentistry, Postgraduate and Research Division, Department of Oral and Maxillofacial Surgery Specialty, National Autonomous University of Mexico, Mexico City, Mexico



BACKGROUND: Oral epithelial dysplasia (OED) is the presence of cells of an abnormal type within a tissue, which may signify a stage preceding the development of cancer. Our aim was to determine the interrelation between the expression of multiple molecular markers and the histological features of oral dysplasia.
METHODS: Fifteen samples of OED (five for each severity degree) were analyzed through software assisted image cytometry nuclear morphology. p53 (wild-type and mutated form), Bax and Bcl2 expression was immunohistochemically determined, and the gene expression of MMP1, MMP2, MMP9 and hTERT was determined by RT-PCR. The mean, standard deviation, ANOVA and Fisher’s Exact Test (P<0.05) were performed.
RESULTS: Our analysis indicated congruence between the software-assisted measurement of nuclear morphology and severity degree. Only five samples were positive to p53-mutated form; and Bax was more expressed than Bcl-2. hTERT expression was significantly expressed in relation to severity, and MMP1 was predominantly expressed, followed by MMP9 and MMP2.
CONCLUSIONS: Our results reinforce that software-assisted measurement is an alternative to severity degree determination. MMP1 is an important marker for severity dysplasia degree; however, the predominant expression of Bax over Bcl-2 suggests that this pro-apoptotic state could be used to minorize the progression, perhaps, as a future therapeutic target.


KEY WORDS: Matrix metalloproteinase 1; hTERT mp30-38 peptide; Genes, p53

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