ORIGINAL ARTICLE   

Minerva Stomatologica 2018 April;67(2):68-73

DOI: 10.23736/S0026-4970.17.04108-5

Copyright © 2017 EDIZIONI MINERVA MEDICA

lingua: Inglese

A comparative evaluation of APF gel, CPP/ACP paste alone and in combination with carbon dioxide laser on human enamel resistance to acid solubility using atomic absorption spectrometry: an in-vitro study

Ali NOZARI ¹, Azade RAFIEE ^{1, 2} ✉, Sara DEHGHAN KHALILI ³, Reza FEKRAZAD ⁴

¹ Department of Pediatric Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran; ² Oral and Dental Disease Research Center, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran; ³ Department of Pediatric, Dental Faculty, AJA University of Medical Sciences, Tehran, Iran; ⁴ Department of Periodontology, Faculty of Dentistry, Laser Research Center in Medical Sciences, AJA University of Medical Sciences, Tehran, Iran

BACKGROUND: The aim of this study was to compare the effects of acidulated phosphate fluoride (APF) gel, calcium phosphopeptide-amorphous calcium phosphate (CPP/ACP) paste alone and in combination with CO2 laser on the resistance of enamel to acid solubility.
METHODS: Ninety enamel sections were obtained from 15 extracted teeth and were randomly assigned to six groups: 1) control group; 2) APF group; 3) CPP-ACP group; 4) CO2 laser group; 5) APF + CO2 group; and 6) CPP-ACP + CO2 group. The specimens were individually demineralized in 0.1 M lactic acid solution with adjusted pH of 4.8 for 24h at 37 ºC. The acid solubility was determined using atomic absorption spectrometry. Statistical analysis was done using one-way ANOVA and Tukey-Kramer post hoc test (P<0.05).
RESULTS: The average extent of calcium ion released (ppm) was estimated as follow: group 1: 6.974±1.757, group 2: 5.363±1.383, group 3: 6.962±1.489, group 4: 6.890±1.560, group 5: 4.803±1.080 and group 6: 6.789±1.218. Based on the between-group comparison results, group 2 and group 5 showed significant differences with the other groups.
CONCLUSIONS: Under the studied conditions, only, the APF group alone and in combination with CO2 laser could decrease enamel acid solubility.

KEY WORDS: Fluorides - Casein phosphopeptide-amorphous calcium phosphate nanocomplex - Lasers, gas - Solubility - Spectrophotometry, atomic