ORIGINAL ARTICLES  NEW TRENDS IN BIOENCAPSULATION - Part 1 

Minerva Biotecnologica 2005 December;17(4):237-43

Copyright © 2006 EDIZIONI MINERVA MEDICA

lingua: Inglese

Optimization of poly(2-(dimethylamino)ethyl methacrylate)-co-poly(ethyleneglycol)/DNA complexes designed for cell transfection

Grandfils C., Emonds-Alt J.

Interfaculty Center of Biomaterials, Ulg Chemistry Institute, Liege Sart-Tilman, Belgium

Cell transfection relies upon the delivery of genomic material within cells in order to express selected DNA sequences. Non-viral gene delivery systems are preferable over viral vectors for several reasons: biosafety, low immunogenicity, higher loading capacity, and easiness of production. Their major drawbacks actually are their limited efficiency in vivo compared to viruses, their cytotoxicity and the fact that they are rapidly cleared up from the bloodstream. The aim of our study was to better characterize the physico-chemical behavior of the polycations based on poly(2-(dimethylamino)ethyl methacrylate)-co-poly(ethyleneglycol) and to control the formulation step to produce the polyelectrolyte complexes.