Home > Riviste > Minerva Biotecnologica > Fascicoli precedenti > Minerva Biotecnologica 2004 June;16(2) > Minerva Biotecnologica 2004 June;16(2):127-33

ULTIMO FASCICOLO
 

JOURNAL TOOLS

eTOC
Per abbonarsi PROMO
Sottometti un articolo
Segnala alla tua biblioteca
 

ARTICLE TOOLS

Estratti
Permessi

 

ORIGINAL ARTICLES  CELLULAR AND MOLECULAR ADVANCES IN THE STUDY OF INFLAMMATION 

Minerva Biotecnologica 2004 June;16(2):127-33

Copyright © 2004 EDIZIONI MINERVA MEDICA

lingua: Inglese

Inositide-modifying enzymes in differentiation of myeloid cells

Bertagnolo V. 1, Brugnoli F. 1, Bovolenta M. 1, Capitani S. 1, 2

1 Signal Transduction Unit, Section of Human Anatomy Department of Morphology and Embryology, University of Ferrara, Ferrara, Italy; 2 MIUR ICSI (Interdisciplinary Center for the Study of Inflammation), University of Ferrara, Ferrara, Italy


PDF


Differentiation and ­response of neu­troph­ils to inflam­ma­to­ry stim­u­li con­sist of a com­plex ­sequence of ­events includ­ing cyto­skel­e­ton remod­el­ling and ­shape chang­es. While a num­ber of stud­ies per­formed ­with dif­fer­ent approach­es ­have indi­cat­ed the involve­ment of PLC and PI 3-K path­ways in a varie­ty of chem­o­at­trac­tant-­induced respons­es, the ­issue of wheth­er ­these inosi­tide-mod­i­fy­ing ­enzymes ­play a ­role ­also in mod­ulat­ing the phe­no­typ­ical mat­u­ra­tion pro­cess of inflam­ma­tion ­cells has not ­been ful­ly ­addressed. Recent ­work per­formed on mye­loid ­cells dem­on­strat­ed ­that the ­ATRA-­induced gra­nu­lo­cyt­ic mat­u­ra­tion of the HL-60 pro­myel­o­cyt­ic ­cell ­line, a ­well stud­ied mod­el of neu­troph­il-­like dif­fe­ren­ti­a­tion, is accom­pa­nied by the accu­mu­la­tion of spe­cif­ic iso­forms of PLC and PI 3-K ­inside the nucle­ar com­part­ment of dif­fer­en­tiat­ed ­cells. In par­tic­u­lar, it has ­been ­found ­that the activ­ity of PI 3-K, ­that ­appears to be essen­tial for the dif­fer­en­tia­tive pro­cess, is depen­dent on its inter­ac­tion ­with ­actin, in ­both cyto­plasm and nucle­ar com­part­ments of HL-60 ­cells. PI 3-K/­actin asso­ci­a­tion ­inside the nucle­us is medi­at­ed by tyro­sine phos­phor­y­lat­ed Vav, ­which may ­then be respon­sible of tar­get­ing PI 3-K to its nucle­ar ­matrix-asso­ciat­ed intra­nu­cle­ar sub­strates. Stemming ­from the ­notion ­that the chang­es in cyto­skel­e­ton assem­bly are reg­u­lat­ed by phos­phoi­no­si­tides, we ­review the ­data con­cern­ing the intra­nu­cle­ar expres­sion and activ­ity of spe­cif­ic inosi­tide-mod­i­fy­ing ­enzymes dur­ing dif­fe­ren­ti­a­tion of tumo­ral mye­loid pre­cur­sors. The col­lect­ed evi­denc­es sug­gest ­that chang­es of the intra­nu­cle­ar ­pool of phos­phoi­no­si­tides ­play key ­roles in the chang­es of the nucle­os­kele­ton ­that char­ac­ter­ize gra­nu­lo­cyt­ic mat­u­ra­tion.

inizio pagina