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Minerva Biotecnologica 2003 September;15(3):185-9

Copyright © 2004 EDIZIONI MINERVA MEDICA

lingua: Inglese

Xenotransplantation of alginate-chitosan-polyethyleneglycol microencapsulated rat islets in the treatment of mice diabetes mellitus. Preliminary data and future perspective

Lou W., Qin X., Wu Z.

Department of General Surgery, Zhong Shan Hospital, FuDan University, Shangai, China


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Aim. To com­pare the ­results of xen­o­tran­splan­ta­tion of Alginate-poly­ly­sine-algi­nate micro­en­cap­su­lat­ed rat ­islets (APA micro­en­cap­su­lat­ed ­group) and algi­nate-chit­o­san-poly­ethy­le­neg­ly­col micro­en­cap­su­lat­ed rat ­islets (ACP micro­en­cap­su­lat­ed ­group) in the treat­ment of ­mice dia­betes mel­lit­us.
Methods. Streptozotosin 220 mg/kg ­intra-abdom­i­nal injec­tion pre­pared the C57BL/6 ­mice dia­bet­ic mod­el. Three ­groups ­were ­assigned: ­naked rat ­islet ­group; APA micro­en­cap­su­lat­ed ­islets trans­plan­ta­tion ­group and ACP micro­en­cap­su­lat­ed ­islets trans­plan­ta­tion ­group; 200 of ­each ­kind of ­islets ­were trans­plant­ed ­into the abdom­i­nal cav­ities of dia­bet­ic ­mice in the respec­tive ­groups. The lev­els of ­blood glu­cose ­were record­ed ­before and ­after the trans­plan­ta­tion.
Results. One day ­after trans­plan­ta­tion, the lev­el of ­blood glu­cose of all ­groups was ­returned to nor­mal. On the 2nd day, the lev­el of ­blood glu­cose of the ­naked ­islet ­group was ­back to the pretrans­plan­ta­tion lev­el, ­while ­that of the APA micro­en­cap­su­lat­ed ­group and the ACP micro­en­cap­su­lat­ed ­group ­were ­kept in the nor­mal ­range for 57±14.61 and 41.67±16.73 ­days ­after trans­plan­ta­tion respec­tive­ly. No dif­fer­ence was ­observed ­between ­these 2 ­groups.
Conclusion. The ACP micro­cap­sule ­could pro­vide ­immune iso­la­tion to ­xeno-­islets as the APA micro­cap­sule ­does; trans­plan­ta­tion of APA micro­en­cap­su­lat­ed rat ­islets ­could ­reverse the dia­bet­ic ­state of ­mice.

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