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Minerva Biotecnologica 2018 June;30(2):51-7

DOI: 10.23736/S1120-4826.18.02372-8


lingua: Inglese

Osteoblast cell behavior on chitosan/poly caprolactone nanifiber for hard tissue engineering application

Fereshteh SHARIFI 1, Seyed M. ATYABI 2 , Dariush NOROUZIAN 2, Mojgan ZANDI 3, Shiva IRANI 1

1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran; 2 Department of NanoBiotechnology, Pasteur Institute of Iran, Tehran, Iran; 3 Department of Biomaterial, Iran Polymer and Petrochemical Institute, Tehran, Iran


BACKGROUND: Electrospinning is a versatile and unique technique which produces fibers with diameters in the range from µm down to nm. This technique can produce fibers with high similarity from aspects of structural and textural to the architecture of natural extracellular matrix (ECM). Therefore, fibers fabricated through electrospinning have great potential for determining cell response in tissue regeneration medicine. Another key factor has unavoidable role is a kind of biomaterial used for fabricating scaffold. One of the most practical and favorable natural biomaterial in biomedical application is chitosan. Although these biomaterials have advantages like: biocompatible, biodegradable and versatile, blending these biomaterials with synthetic biopolymer such as poly caprolactone (PCL) may be improve and enhance their stability and mechanical properties. In this literature, we studied the effect of various percentages of natural biomaterial blending with PCL on human osteoblast cells (MG63) behavior.
METHODS: Blending chitosan-PCL was fabricated through electrospinning technique. Nano-fibers morphology was evaluated by scanning electron microscope (SEM). Scaffold biocompatibility and proliferation rate were assessed using inverted microscope and MTT test, respectively.
RESULTS: As expected, the result of SEM image exhibited suitable and applicable fiber morphology. According to biocompatible experiments, designed scaffolds had suitable condition for using in biomedical regeneration. Afterward, the graph obtained from MTT assays showed that the proliferation rate of MG63 on chitosan-PCL 10% scaffold significantly better than other chitosan-PCL percentages and control group.
CONCLUSIONS: Our results demonstrated that blending chitosan-PCL would be creating condition and situation with highest similarity to the natural ECM.

KEY WORDS: Tissue engineering - Chitosan - Osteoblasts

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