ORIGINAL ARTICLES   

Minerva Biotecnologica 2016 December;28(4):187-92

Copyright © 2016 EDIZIONI MINERVA MEDICA

lingua: Inglese

Fibroblast cell behavior study on chitosan/laminin scaffolds for tissue engineering

Seyed M. ATYABI ¹, Saye ATOON ², Shiva IRANI ², Mohammad T. KHORASANI ³, Morteza D. JOUPARI ⁴

¹ Department of Pilot Biotechnology, Pasteur Institute, Tehran, Iran; ² Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran; ³ Department of Biomaterials, Iranian Polymer and Petrochemical Institute, Tehran, Iran; ⁴ Department of Animal and Marine Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

BACKGROUND: Tissue engineering is an exciting research region that aims at regenerative alternatives to harvested tissues for transplantation. Scaffolds provide essential support as artificial extracellular matrix (ECM), which allows cells to growth and proliferate. Three-dimensional porous chitosan scaffolds are attractive candidates for tissue engineering applications. Combination of chitosan with laminin as a natural polymer seems to improve the cell growth. In this study, chitosan/laminin was exploited as scaffold for tissue engineering.
METHODS: Freeze-drying techniques was used to fabricate chitosan/laminin nanocomposites for L929 fibroblast cells seeding, proliferation and attachment. The physico-chemical properties of the scaffold were fully characterized by using scanning electron microscopy (SEM). Consequently, the biocompatibility of scaffold was evaluated using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide) assay. For the examination of nuclear morphology, cells were stained with 2 μg/mL of 4’, 6-diamidino-2-phenylindole (DAPI) to stain the DNA, and observed under a fluorescence microscope.
RESULTS: Furthermore, microstructure observation with SEM suggests the formation of cylindrical-shaped porous structure and interconnected porosity so that cells adhesion within the structure is well in depth. Cell cultural test showed that the nano-sized chitosan/laminin scaffolds are nontoxic and biocompatible which can promote proliferation and attachment of fibroblast cells.
CONCLUSIONS: It seems that the nanocomposite can be suitable for improved tissue engineering applications.