ORIGINAL ARTICLES   

Minerva Biotecnologica 2012 June;24(2):29-36

Copyright © 2012 EDIZIONI MINERVA MEDICA

lingua: Inglese

Antibacterial potential of bioactive compounds from fermented culture of Pseudomonas aeruginosa SRM1 against Xanthomonas oryzae pv. Oryzae

Kulshreshtha G., Velusamy P.

Department of Biotechnology, School of Bioengineering, SRM University, Chennai, India

Aim. The aim of the study was to investigate the antibacterial activity of soil bacterium against rice bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae (Xoo).
Methods. Among the isolated bacteria, strain SRM1 exhibited strong antagonistic activity, and was identified as Pseudomonas aeruginosa SRM1 based on morphological observation and 16S rRNA analysis. Besides having antibacterial activity, two biologically active compounds were isolated from fermented culture of P. aeruginosa SRM1 and were found to be benzoic acid (BA) and phenylacetic acid (PAA) based on their gas chromatography-electron impact-mass spectrometry (GC-EI-MS) and trimethylsilation GC-EI-MS data. The isolated compounds were further evaluated for antimicrobial activities against different strains of pathogenic bacteria and fungus which were based on the minimal inhibitory concentration (MIC) by disc diffusion method.
Results. Results from this study showed that PAA had a higher level of antimicrobial activity against all tested pathogens at lower MIC as compared to other compound BA and reference drug chloramphenicol.
Conclusion. This study suggests that product of bioactive compounds from the P. aeruginosa SRM1 can be effectively used as potent antimicrobial agents.