ORIGINAL ARTICLES   

Minerva Biotecnologica 2006 December;18(4):159-64

Copyright © 2006 EDIZIONI MINERVA MEDICA

lingua: Inglese

A collection of expressed sequence tags (ESTs) from totipotent cDNA of durum wheat

Cifarelli R. A., D’Onofrio O., Lauria G., Gallitelli M., Cellini F.

Metapontum Agrobios, Metaponto (MT), Italy

Aim. This study aims to the development of a database to store our expressed sequence tags (ESTs). The sequencing of a cDNA library to generate ESTs is an effective tool for research activities such as gene discovery, generation of molecular markers and characterization of transcription patterns. In this issue we report a sequence analysis of a durum wheat cDNA library.
Methods. In order to maximize the mRNAs representativeness, a patented method is used, which allows the preparation of a totipotent cDNA library from durum wheat regardless of the developmental stage of the material from which the library is originated.
Results. To date, we have archived 11 000 ESTs and 7 648 of them were subjected to both a BLASTX and BLASTN search.
Conclusion. When compared to public databases, 94%, 1% and 5% of the ESTs sequences showed similarity, respectively, to genes from Triticum aestivum, Triticum turgidum and other Triticeae, approximately other organisms and no match to known sequences. Moreover, we created a functional inventory of these sequences based on the BLASTX annotation.