ORIGINAL ARTICLES  MICROARRAY MEETING 2003: III CONVEGNO ITALIANO SULLA TECNOLOGIA
Segrate (MI), 9-10 Giugno 2003 

Minerva Biotecnologica 2003 December;15(4):235-44

Copyright © 2004 EDIZIONI MINERVA MEDICA

lingua: Inglese

Application of a cDNA microarray for the analysis of muscular dystrophies and childhood leukemias

Campanaro S. ¹, De Pittà C. ¹, Celegato B. ¹, Millino C. ¹, Romualdi C. ¹, Pacchioni B. ¹, Trevisan S. ¹, Bellin M. ¹, Cagnin S. ¹, Tombolan L. ¹, Fanin M. ², Pegoraro E. ², TE Kronnie G. ³, Pescatori M. ⁴, Valle G. ¹, Basso G. ³, Ricci E. ⁴, Angelini C. ², Lanfranchi G. ¹

¹ CRIBI Biotechnology Center and Departement of Biology, University of Padua, Padua, Italy; ² Department of Neurosciences, University of Padua, Padua, Italy; ³ Department of Pediatrics, University of Padua, Padua, Italy; ⁴ Institute of Neurology, Catholic University, Rome, Italy and UILDM-Centre for Neuromuscular Diseases, Rome, Italy

Aim. Microarray tech­nique can meas­ure the expres­sion of thou­sands of ­genes simul­ta­ne­ous­ly and can iden­ti­fy sub­tle chang­es in expres­sion ­between dif­fer­ent bio­log­i­cal ­states.
Methods. Using our cDNA col­lec­tion ­obtained ­from system­at­ic sequenc­ing of cDNA librar­ies ­from skel­e­tal mus­cle, ­heart and ­bone mar­row tis­sues, we ­have devel­oped a micro­ar­ray com­posed of 4 670 sequenc­es cor­re­spond­ing to the 400-500 bp ­region locat­ed at the 3’-end of cDNA tran­scripts.
Results. We ­used ­this micro­ar­ray plat­form in ­order to inves­ti­gate the expres­sion pro­file in 4 dif­fer­ent pathol­o­gies: ­limb gir­dle mus­cu­lar dys­tro­phy ­type 2B (­LGMD 2B), facios­ca­pu­lo­hu­me­ral­ mus­cu­lar dys­tro­phy (­FSHD1A), con­gen­i­tal mus­cu­lar dys­tro­phy (CMD) and a ­group of child­hood leu­kae­mi­as. Our aim is to com­pare dif­fer­ent pathol­o­gies in ­order to iden­ti­fy sig­nif­i­cant ­genes ­whose expres­sion char­ac­ter­ize ­each sin­gle dis­ease.
Conclusion. The cDNA micro­ar­ray plat­form devel­oped in our labor­a­to­ry ­allow the iden­tifi­ca­tion of dif­fe­ren­tial­ly ­expressed ­genes in mus­cu­lar dys­tro­phies and in child­hood leu­kae­mi­as in ­order to bet­ter ­define the mole­cular mech­a­nism of ­these pathol­o­gies. Moreover our micro­ar­ray experi­ments iden­ti­fy dif­fer­ent ­forms of the ­same pathol­o­gy on the ­base of the tran­scrip­tion­al pro­file.