Home > Riviste > Minerva Biotechnology and Biomolecular Research > Fascicoli precedenti > Minerva Biotecnologica 2003 June;15(2) > Minerva Biotecnologica 2003 June;15(2):129-36

ULTIMO FASCICOLO
 

JOURNAL TOOLS

Opzioni di pubblicazione
eTOC
Per abbonarsi
Sottometti un articolo
Segnala alla tua biblioteca
 

ARTICLE TOOLS

Estratti
Permessi
Share

 

ORIGINAL ARTICLES  TRENDS IN MOLECULAR DIAGNOSIS AND THERAPY OF β-THALASSEMIA AND SICKLE CELL ANEMIA 

Minerva Biotecnologica 2003 June;15(2):129-36

Copyright © 2003 EDIZIONI MINERVA MEDICA

lingua: Inglese

The use of cell culture procedures for studying fetal hemoglobin stimulating drugs

Fibach E.

Department of Hematology, Hadassah University Hospital, Jerusalem, Israel


PDF


High ­fetal hemo­glo­bin (HbF) has ­been ­shown to ame­li­o­rate the clin­i­cal symp­toms of ­patients ­with β-hemo­glob­i­nop­a­thies, β-tha­las­se­mia (β-­thal) and sick­le ­cell ane­mia (SCA). Research is there­fore ­focus on find­ing ­drugs ­capable of reac­ti­vat­ing the γ-glo­bin ­genes and stim­u­lat­ing the pro­duc­tion of HbF. Several in ­vitro experi­men­tal mod­els ­have ­been devel­oped to ­serve ­this pur­pose. Two mod­els are the sub­ject of ­this ­review: in ­vitro estab­lished eryth­roid-­like ­cell ­lines and pri­mary cul­tures of eryth­roid ­cells ­derived ­from pro­gen­i­tors ­obtained ­from nor­mal ­donors and ­patients ­with β-­thal and SCA. These experi­men­tal mod­els are use­ful for screen­ing of com­pounds and for stud­y­ing ­their mech­a­nism of ­action at the cel­lu­lar and molec­u­lar lev­els. These stud­ies are essen­tial for find­ing, test­ing and devel­op­ing new, effec­tive and ­safe ­drugs.

inizio pagina