ORIGINAL ARTICLES  TRENDS IN MOLECULAR DIAGNOSIS AND THERAPY OF β-THALASSEMIA AND SICKLE CELL ANEMIA 

Minerva Biotecnologica 2003 June;15(2):93-7

Copyright © 2003 EDIZIONI MINERVA MEDICA

lingua: Inglese

Recent advances in molecular diagnosis using surface plasmon resonance and biosensor technology for detection of β-thalassemia mutations

Breveglieri G. ^{1, 2}, Gardenghi S. ², Gambari R. ^{1, 2}, Feriotto G. ¹

¹ Biotechnology Center, University of Ferrara, Ferrara, Italy; ² Department of Biochemistry and Molecular Biology, University of Ferrara, Ferrara, Italy

In the ­present ­review we ­describe a new meth­o­dol­o­gy employ­ing sur­face plas­mon res­o­nance (SPR) and bio­sen­sor tech­nol­o­gy to ­detect ­point muta­tions, includ­ing ­those caus­ing a ­severe ­form of β°­IVSI-1 tha­las­se­mia. The ­data pre­sent­ed ­allow to con­clude ­that bio­spe­cif­ic inter­ac­tion anal­y­sis (BIA) is a ­fast and auto­mat­able ­approach for detect­ing muta­tions of the β-glo­bin ­gene by the ­real-­time mon­i­tor­ing of hybrid­iza­tion ­between oli­go­nu­cleo­tide (ODN) ­probes and tar­get bio­tin­y­lat­ed poly­me­rase-­chain reac­tion (PCR) prod­ucts gen­er­at­ed ­from genom­ic DNA of nor­mal, het­er­o­zy­gous sub­jects and homo­zy­gous β°-tha­las­se­mia ­patients and immob­il­ized on strep­tav­i­din coat­ed sen­sor ­chips. During the asso­ci­a­tion ­phase, dis­crim­i­na­tion ­between mis­matched and ­full ­matched ODN/PCR prod­uct ­hybrids is read­i­ly and repro­du­cibly ­observed.