MICROARRAY MEETING 2002: NEW DEVELOPMENTS IN MUTATION DETECTION AND GENE EXPRESSION
Segrate, MI (Italy), April 12, 2002 

Minerva Biotecnologica 2002 December;14(3-4):231-6

Copyright © 2003 EDIZIONI MINERVA MEDICA

lingua: Inglese

A review of strategies based on primer extension (PEX) technology

Gemiani F. ¹, Landi S. ^{1, 2}, Canzian F. ¹

¹ Genome Analysis Group, International Agency for Research on Cancer, Lyon, France; ² Dipartimento di Scienze dell’Uomo e dell’Ambiente, University of Pisa, Pisa, Italy

In prim­er exten­sion (PEX) reac­tion, a DNA poly­me­rase is ­used to ­extend spe­cif­i­cal­ly a prim­er ­that ­anneals imme­di­ate­ly adja­cent to the var­i­ant nucle­o­tide. The reac­tion ­allows high­ly spe­cif­ic detec­tion of ­point muta­tions and sin­gle nucle­o­tide poly­mor­phisms (­SNPs). ­Because all ­SNPs can be ana­lyzed ­with ­high spec­i­fic­ity at the ­same reac­tion con­di­tions, PEX is a prom­is­ing reac­tion prin­ci­ple for mul­ti­plex ­high-through­put gen­o­typ­ing ­assays. ­This ­review dis­cuss­es the pos­sible appli­ca­tions of PEX and dif­fer­ent approach­es of prim­er exten­sion reac­tions.