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INTERNATIONAL ANGIOLOGY

Rivista di Angiologia


Official Journal of the International Union of Angiology, the International Union of Phlebology and the Central European Vascular Forum
Indexed/Abstracted in: BIOSIS Previews, Current Contents/Clinical Medicine, EMBASE, PubMed/MEDLINE, Science Citation Index Expanded (SciSearch), Scopus
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International Angiology 2018 Apr 11

DOI: 10.23736/S0392-9590.18.03987-1

Copyright © 2018 EDIZIONI MINERVA MEDICA

lingua: Inglese

Elevated extracellular nucleosomes and their relevance to inflammation in stage 5 chronic kidney disease

Trung PHAN 1, Ryan MCMILLAN 1, Leonidas SKIADOPOULOS 1, Amanda WALBORN 1, Debra HOPPENSTEADT 2, Jawed FAREED 2 , Vinod BANSAL 3

1 Stritch School of Medicine, Loyola University of Chicago, Maywood, IL, USA; 2 Unit of Hemostasis and Thrombosis, Department of Pathology, Loyola University Medical Center, Maywood, IL, USA; 3 Unit of Nephrology, Department of Internal Medicine, Loyola University Medical Center, Maywood, IL, USA


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BACKGROUND: Chronic kidney disease is a disorder characterized by a pro-inflammatory state that is associated with increased morbidity and mortality. Endogenous damage- associated molecular patterns, including nucleosomes, may contribute to this persistent inflammation. The aim of this study was to profile and evaluate the clinical significance of circulating nucleosomes in patients with Stage 5 chronic kidney disease (CKD5) on hemodialysis (HD).
METHODS: Under institutional review board approval, plasma samples were collected from 90 CKD5-HD patients (45 male and 45 female) prior to hemodialysis. Normal human plasma samples (25 male and 25 female) were used as a control group. Commercial enzyme-linked immunosorbent and colorimetric assays were used to profile nucleosome levels and biochemical markers of kidney injury, inflammation, thrombosis, and renal function in CKD5-HD and control groups. Clinical laboratory parameters were documented from the electronical medical record and correlated to nucleosome levels in the CKD5-HD cohort.
RESULTS: In comparison to healthy volunteers, the plasma from CKD5-HD patients exhibited markedly elevated nucleosomes (P < 0.0001). Furthermore, nucleosome levels correlated with WBC count (P = 0.025, R = 0.243) and CRP (P = 0.019, R = 0.266) levels. No correlation was found between nucleosomes and the other parameters studied.
CONCLUSIONS: Our findings indicate extracellular nucleosomes are significantly elevated in CKD5-HD, suggesting increased cell death and/or inflammation. The observed correlations between nucleosomes and parameters of inflammation is suggestive of a complex, systemic inflammatory process underlying renal deterioration, consistent with the literature. Thus, nucleosomes may play a role in the pathogenesis and outcome of CKD5-HD.


KEY WORDS: Chronic kidney disease - Chronic renal insufficiency - End-stage renal disease - Nucleosomes - Inflammation

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Publication History

Article first published online: April 11, 2018
Manuscript accepted: April 4, 2018
Manuscript received: February 23, 2018

Per citare questo articolo

Phan T, Mcmillan R, Skiadopoulos L, Walborn A, Hoppensteadt D, Fareed J, et al. Elevated extracellular nucleosomes and their relevance to inflammation in stage 5 chronic kidney disease. Int Angiol 2018 Apr 11. DOI: 10.23736/S0392-9590.18.03987-1

Corresponding author e-mail

jfareed@luc.edu