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Original articles   

International Angiology 2010 August;29(4):331-7


lingua: Inglese

High MAPK p38 activity and low level of IL-10 in intermittent claudication as opposed to stable angina

El Marghani A. M. 1, Abuabaid H. M. 2, Hurtig-Wennlöf A. 2, Sirsjö A. 2, Norgren L. 3, Kjellen P. 1

1 School of Science and Technology, Örebro University, Örebro, Sweden; 2 School of Health and Medical Sciences, Örebro University, Örebro, Sweden; 3 Department of Surgery, Örebro University Hospital, Örebro, Sweden


AIM: The aim of the present pilot study was to relate the activity of MAPK p38 with the levels of pro- and anti-inflammatory cytokines in a small cohort of patients with either stable angina (N=5) or intermittent claudication (N=5) compared to healthy controls (N=10).
METHODS: The activity of MAPK p38 was determined in peripheral blood mononuclear cells, isolated from whole blood by western blot using phospho-specific anti-MAPK p38 antibodies. Cytokine levels of 11 pro- and anti-inflammatory cytokines were determined from the serum using flow cytometry.
RESULTS: We found a significant elevation of the MAPK p38 activity in the intermittent claudication group (P=0.0027) compared with the healthy control group whereas the stable angina group showed similar MAPK p38 activity as the healthy control group. The IL-10 level in serum found in the stable angina group was significantly higher compared with both the healthy control group (P=0.0116) and the intermittent claudication group (P=0.0317).
CONCLUSION: Our results imply that there is a casual relationship between increased levels of the anti-inflammatory cytokines IL-10 and IL-4 and the activity of the MAPK p38. Possibly has IL-10 a protective role that down-regulates the activity of MAPK p38 and thereby further inflammatory processes in stable angina patients.

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