ORIGINAL ARTICLES   

International Angiology 2000 June;19(2):142-6

Copyright © 2001 EDIZIONI MINERVA MEDICA

lingua: Inglese

Smooth muscle cell migration induced by shear-loaded platelets and endothelial cells. Enhanced platelet-derived growth factor production by shear-loaded platelets

Nakazawa T., Yasuhara H., Shigematsu K., Shigematsu H.

From the Division of Vascular Surgery, Department of Surgery, The University of Tokyo, Tokyo, Japan

Back­ground. Uncon­trolled migra­tion and pro­life­ra­tion of ­smooth mus­cle cells (SMC) are the main mech­a­nisms of devel­op­ment of ath­e­ros­cle­rot­ic neo­in­ti­ma. How­ev­er, it has not yet been elu­ci­dat­ed how mechan­i­cal ­stress is ­involved in this pro­cess. We inves­ti­gat­ed smooth mus­cle cell mit­o­gen­ic activ­ity ­induced by shear-load­ed plate­lets and endo­the­lial cells.
Meth­ods. Experi­men­tal ­design: in vitro experi­men­tal study. We ­devised four types of con­di­tioned media; super­na­tant of mixed cul­ture of plate­lets and endo­the­lial cell (ST), super­na­tant of shear-load­ed mixed cul­ture of plate­lets and endo­the­lial cell (SH), ST medi­um neu­tral­ised with anti-PDGF anti­body (ST+), and SH medi­um neu­tral­ised with anti-PDGF anti­body (SH+). ­Smooth mus­cle cells were cul­tured in each con­di­tioned medi­um, and their spread­ing activ­ity was deter­mined under a micro­scope.
­Results. ­Smooth mus­cle cells spread­ing activ­ity in the SH group was sig­nif­i­cant­ly great­er than that in the ST group. Their spread­ing activ­ity was sup­pressed by anti-PDGF anti­body under shear con­di­tions (SH+), but it was not by anti-PDGF anti­body under stat­ic con­di­tions (ST+).
Con­clu­sions. Our ­results dem­on­strate that plate­let-­derived ­growth fac­tor is pro­duced by shear-load­ed plate­lets and endo­the­lial cells, and local mechan­i­cal forc­es may play an impor­tant role in car­di­o­vas­cu­lar dis­ease.