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Gazzetta Medica Italiana - Archivio per le Scienze Mediche 2022 April;181(4):271-7

DOI: 10.23736/S0393-3660.21.04735-5

Copyright © 2021 EDIZIONI MINERVA MEDICA

lingua: Inglese

Dynamization-induced nanosolutes (DINS) in ultra-dilute solutions. Analysis on homeopathic dilute capsules below and over the Avogadro’s number

Alfonso TRAMONTANA 1 , Eleonora NICOLAI 2, Roberto SORGE 3, Roberta GAZIANO 2, Daniele MARINO 2, Giovanni MONTELEONE 4, Juan C. MIANGOLARRA PAGE 1, Nicola ROSATO 2

1 Department of Physical and Rehabilitation Medicine, University Rey Juan Carlos, Móstoles, Madrid, Spain; 2 Department of Experimental Medicine, Tor Vergata University, Rome, Italy; 3 Department of Systems Medicine, Faculty of Medicine and Surgery, Tor Vergata University, Rome, Italy; 4 Department of Biomedicine and Preventive Medicine, Faculty of Medicine and Surgery, Tor Vergata University, Rome, Italy



BACKGROUND: Homeopathy is a controversial topic. It is not clear what is the role of the succussion that should increase the efficacy of the homeopathic remedy. The primary aim is to verify whether could be the presence of nanosolutes when the dilution increases past the Avogrado’s number and whether the succussion process increases the presence of nanosolutes. The secondary goal is to provide a rational model to develop theories on the mechanism of action of these solutions from a clinical standpoint.
METHODS: Three different solution lines of highly diluted solutions have been made for each active ingredient: the first line was a non-shaken solution, the other two lines were shaken. They have run the following measurements: Fluorescence, Absorption, Dynamic Light Scattering, Microbiological analysis.
RESULTS: Fluorescence, light scattering and absorption, did not show any difference between the samples containing only water and samples derived from the solutions containing the active ingredients The shaken dilutions have shown a higher content in bacteria than the corresponding non-shaken (Biesse Bioscreen measurement: 0.000 ANOVA and Bonferroni). The bacteria are largely gram-positive at difference with those presence in the reference water solution, that are largely gram-negative.
CONCLUSIONS: This study allows highlighting the presence of nanosolutes in high-diluted solutions Such nanosolutes are bacteria, which would bond with parts of parent substance in clusters. The succussion increases the number of nanosolutes. It is possible to hypothesize a mechanism of action of the homeopathic remedy on microbiotic basis.


KEY WORDS: Homeopathy; Complementary therapies; Pharmaceutical solutions

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