ORIGINAL ARTICLE   

Gazzetta Medica Italiana - Archivio per le Scienze Mediche 2019 October;178(10):741-8

DOI: 10.23736/S0393-3660.18.03910-4

Copyright © 2018 EDIZIONI MINERVA MEDICA

lingua: Inglese

The expression of calcium-sensing receptor during rotavirus induced diarrhea in neonatal mice

Haohai HUANG ¹ ✉, Yong PU ², Dan LIAO ³, Zhu ZHU ^{4, 5}, Jian WANG ^{4, 5}, Yejia CUI ²

¹ Department of Clinical Pharmacy, Dongguan Third People’s Hospital, Affiliated Dongguan Shilong People’s Hospital of Southern Medical University, Dongguan, China; ² Department of Clinical Laboratory, Dongguan Third Peoples Hospital, Affiliated Dongguan Shilong People’s Hospital of Southern Medical University, Dongguan, China; ³ Department of Gynecology, Dongguan Third People’s Hospital, Affiliated Dongguan Shilong People’s Hospital of Southern Medical University, Dongguan, China; ⁴ Sino-American Cancer Research Institute, Guangdong Medical University, Dongguan, China; ⁵ Scientific Research Platform, Guangdong Medical University, Dongguan, China

BACKGROUND: Studies have shown that rotavirus (RV) induced secretory diarrhea results from a combination of excessive secretion of fluid and electrolytes into the intestinal lumen and reduced fluid absorption. Calcium-sensing receptor (CaSR), a calcium-binding G protein-coupled receptor, is expressed in both absorbing surface cells and secreting crypts of the intestine. Previous studies have revealed that CaSR play an important role in regulating fluid secretion and absorption in acute diarrheal illness. However, whether the CaSR involved in the development of RV-induces diarrhea have not been explored.
METHODS: We focus on the expression levels of CaSR during RV induced diarrhea by establishing a RV SA-11 infected neonatal mouse model. The CaSR mRNA and protein expression were detected by real-time RT-PCR and western blotting, respectively.
RESULTS: Thirty-two of 36 mice in the RV-infected group developed diarrhea and mild dehydration from days 2 to 5 post infection and viral shedding was observed from days 1 to 6. The peak of rotavirus replication was observed at 3 days postinfection. Histological changes of intestinal epithelial cells were characterized by the accumulation of cytoplasmic vacuolation, malaligned villi, and atrophy in the RV-infected group. Our results demonstrated that CaSR mRNA and protein expression were significantly attenuated in the basolateral membrane of colon epithelial cells in RV induced diarrhea group. In addition, we also found that down-regulation of CaSR are associated with the severity of diarrhea.
CONCLUSIONS: These findings suggest that CaSR may play a role in the pathogenesis of RV induced diarrhea by mediating fluid secretion and absorption.

KEY WORDS: Receptors, calcium-sensing; Diarrhea; Rotavirus