Home > Riviste > Gazzetta Medica Italiana Archivio per le Scienze Mediche > Fascicoli precedenti > Gazzetta Medica Italiana Archivio per le Scienze Mediche 2018 December;177(12) > Gazzetta Medica Italiana Archivio per le Scienze Mediche 2018 December;177(12):683-8



Opzioni di pubblicazione
Per abbonarsi
Sottometti un articolo
Segnala alla tua biblioteca


Publication history
Per citare questo articolo



Gazzetta Medica Italiana Archivio per le Scienze Mediche 2018 December;177(12):683-8

DOI: 10.23736/S0393-3660.17.03671-3


lingua: Inglese

Global DNA methylation is stable across time and following acute exercise

James W. NAVALTA 1 , Mihaela A. CIULEI 2, Ramires A. TIBANA 3, Fabrício A. VOLTARELLI 3, Jonato PRESTES 4, John C. YOUNG 1

1 Department of Kinesiology and Nutrition Sciences, University of Nevada, Las Vegas, NV, USA; 2 Department of Nutritional Sciences, Pennsylvania State University, University Park, PA, USA; 3 Department of Physical Education, Federal University of Mato Grosso, Cuiabá, Mato Grosso, Brazil; 4 Department of Health Sciences and Technologies, Catholic University of Brasilia, Brasilia, Federal District, Brazil

BACKGROUND: Elevated global DNA methylation (gDNAm) has been linked to increased risk of cardiovascular disease. It is unknown whether gDNAm is stable across time, and whether it is affected by acute exercise. The purpose was to determine the variation of gDNAm over time, and following acute aerobic and anaerobic exercise.
METHODS: Fourteen participants provided resting samples each week for four consecutive weeks, at two months, and again at three months. Participants completed a maximal aerobic test to exhaustion (VO2max) as well as a series of Wingate anaerobic cycle tests, providing samples before (pre) and after (post) bouts. DNA was isolated, and methylation was analyzed using a commercially available kit. Variation over time was analyzed using a one-way ANOVA, while differences referent to acute exercise was analyzed using a 2(time) x 2(exercise type) repeated measures ANOVA. Significance was accepted at P≤0.05.
RESULTS: gDNAm did not vary over time (P=0.105). Additionally, acute aerobic exercise to maximal exertion did not alter gDNAm (P=0.71; pre 1.9±1.0%, post = 1.5±0.8%), and neither did acute anaerobic exercise (P=0.97; pre = 1.9±1.0%, post = 2.1±1.1%).
CONCLUSIONS: These findings provide evidence that the epigenomic marker gDNAm is a stable measure that does not vary over several months. Additionally, this measure is unaffected by acute exercise perturbations whether aerobic or anaerobic in nature. As gDNAm is inversely associated with aerobic fitness, it would be of interest to determine the time course necessary for chronic adaptations due to exercise training.

KEY WORDS: Epigenomics - Exercise - DNA methylation

inizio pagina