ORIGINAL ARTICLE   

Gazzetta Medica Italiana Archivio per le Scienze Mediche 2017 October;176(10):521-7

DOI: 10.23736/S0393-3660.16.03392-1

Copyright © 2016 EDIZIONI MINERVA MEDICA

lingua: Inglese

Satellite cell content in slow- and fast-twitch muscles after ischemia/reperfusion in mice

Chang-Hyun LIM ¹, Hee-Jeong SON ¹, Shin-Young KIM ², Chang-Keun KIM ¹, Hyo-Jeong KIM ¹ ✉

¹ Department of Sport and Healthy Ageing and Human Physiology, Korea National Sport University, Seoul, South Korea; ² Department of Physical Therapy, Ansan University, Ansan, South Korea

BACKGROUND: Prolonged ischemia can cause the overall death of skeletal muscle fibers. Ischemia/reperfusion (IR) has been widely studied for treatment of injured tissues in different organs.
METHODS: The present study examined the effect of ischemia/reperfusion (IR) on satellite cells (SCs) and myonuclear content in the soleus (SOL) and extensor digitorum longus (EDL) muscles. Twelve-week-old male mice were divided into three groups: and experimental group (measurements at 0.5, 2, 4, 8, 16, and 24 hours of recovery after IR), a sham group and a control group.
RESULTS: The current study demonstrated that the ratio of Ki67 to CD56 in both SOL and EDL muscles increased significantly in early phase of recovery from sham control and decreased again at 2hr perfusion to baseline. Pax7+ cells per fiber and Pax7+ cells to myonuclear number in SOL muscle were unchanged after IR, but these increased slightly after IR in EDL. The number of myonuclei per fiber and the ratio of central nuclei to myonuclear number in both SOL and EDL muscles after IR were unchanged after 24 hours when compared to the sham control. The myonuclear domain was slightly reduced in response to IR in both SOL and EDL muscles.
CONCLUSIONS: A further understanding of how the factors actually function is likely to provide more effective methods of treating injured muscles from IR.

KEY WORDS: Myocardial reperfusion - Skeletal muscle satellite cells - Slow-twitch muscle fibers - Fast-twitch muscle fibers - Reperfusion injury - Cerebellar nuclei