Home > Riviste > The Journal of Cardiovascular Surgery > Fascicoli precedenti > The Journal of Cardiovascular Surgery 2002 December;43(6) > The Journal of Cardiovascular Surgery 2002 December;43(6):869-75

ULTIMO FASCICOLO
 

JOURNAL TOOLS

eTOC
Per abbonarsi PROMO
Sottometti un articolo
Segnala alla tua biblioteca
 

ARTICLE TOOLS

Estratti

 

ORIGINAL ARTICLES  VASCULAR SECTION 

The Journal of Cardiovascular Surgery 2002 December;43(6):869-75

Copyright © 2009 EDIZIONI MINERVA MEDICA

lingua: Inglese

Lipo-prostaglandin E1 is absorbed by vascular smooth muscle cells and may enhance re-endothelialization of vein grafts

Akasaka N., Yamazaki K., Ishikawa M., Koya A., Hirasawa M., Inaba M., Goh K., Sasajima T.

Department of Surgery, Asahikawa Medical University, Asahikawa, Japan


PDF


Background. Enhance­ment of re-en­do­the­lial­iza­tion ­inhibits the pro­gres­sion of ­intimal hyper­plasia. We inves­ti­gated ­uptake of Lip­o­pros­ta­glandin E1 (­LPGE1) by endo­the­lial ­cells (ECs) and vas­cular ­smooth ­muscle ­cells (­VSMCs), and ­effects of the ­LPGE1 on re-en­do­the­lial­iza­tion of ­vein ­grafts.
­Methods. Pri­mary cul­tured ECs and ­VSMCs ­were ­obtained ­from mon­grel ­dogs, and incu­bated in ­mediums con­taining 0.5, 5, 50, and 250 ng/ml of DiI-­LPGE1 (­DLPGE1); its ­uptake was vis­u­al­ized by the stan­dard rhod­a­mine exci­ta­tion, and ­assessed by ­flow cytom­etry. In an implan­ta­tion ­study, the bilat­eral ­femoral ­veins of 18 ani­mals ­were ­implanted ­into the ­femoral ­artery, and the ani­mals ­were ­given 0.2 μg/kg ­LPGE1 for 2 ­weeks. Per­cent­ages of EC ­coverage ­area of the ­grafts (%EC) ­were meas­ured by ­silver ­nitrate ­staining at inter­vals of 3, 7, and 21 ­days ­after implan­ta­tion.
­Results. ­VSMCs ­showed sig­nif­i­cant ­uptake in all of the ­mediums con­taining ­DLPGE1, ­whereas the ECs ­revealed no flu­o­res­cence. In ­flow cytom­etry, his­to­grams of ­VSMCs ­showed a spe­cific ­notch of ­DLPGE1, ­which ­increased the ­height ­according to the ­grade of the con­cen­tra­tions. The ­notch did not ­appear in the his­to­grams of the ECs incu­bated ­with any con­cen­tra­tion nor in the ­VSMCs incu­bated in con­trol ­medium. ­These ­results sug­gested ­that ­LPGE1 is pre­dom­i­nantly ­absorbed by the ­VSMCs and ­exuded PGE1 ­then ­acts on the ­VSMCs ­itself and on ECs ­alike. The %EC at 7 ­days was 58.4±1.9% in the ­DLPGE1 ­group and 31.6±6.8% in con­trols (p<0.05) ­showing a sig­nif­i­cant ­enhancing ­effect of ­DLPGE1 on re-en­do­the­lial­iza­tion of the ­vein ­grafts.
Con­clu­sions. In an ­animal ­model, ­daily admin­is­tra­tion of ­LPGE1 ­resulted in sig­nif­i­cant enhance­ment of ­vein ­graft re-en­do­the­lial­iza­tion. ­LPGE1 was ­absorbed by the ­VSMCs, ­whereas the ECs ­showed no ­uptake, there­fore the enhance­ment is prob­ably due to a par­a­crine ­effect of ­VSMCs.

inizio pagina