ORIGINAL ARTICLE   

Giornale Italiano di Dermatologia e Venereologia 2020 December;155(6):772-4

DOI: 10.23736/S0392-0488.18.06161-8

Copyright © 2018 EDIZIONI MINERVA MEDICA

lingua: Inglese

Lack of detection of Cutavirus DNA using PCR real time in cutaneous T-cell lymphomas

Massimiliano BERGALLO ¹ ✉, Valentina DAPRÀ ¹, Paolo FAVA ², Renata PONTI ², Cristina CALVI ¹, Maria T. FIERRO ², Pietro QUAGLINO ², Ilaria GALLIANO ¹, Paola MONTANARI ¹

¹ Unit of Infectious Diseases, Department of Pediatrics, Regina Margherita Children’s Hospital, University of Turin, Turin, Italy; ² Dermatology Section, Department of Medical Sciences, University of Turin, Turin, Italy

BACKGROUND: A novel human protoparvovirus named Cutavirus has been discovered. We investigated the presence of Cutavirus in a sample of Cutaneous T-cell lymphomas by using PCR real time TaqMan® (Thermo Fisher Scientific, Waltham, MA, USA).
METHODS: In total, 55 CTCL samples were analyzed using a TaqMan® Real time PCR on a 7500 ABI instrument. All of these shown internal control amplification.
RESULTS: The presence of Cutavirus DNA corresponding was examined. CuV DNA sequences were not detected in any skin specimen.
CONCLUSIONS: The role of Cutaviruses in cutaneous cancers remains to be investigated.

KEY WORDS: Parvovirus; Lymphoma, T-cell, cutaneous; Mycosis fungoides; Sézary Syndrome