ORIGINAL ARTICLES   

Giornale Italiano di Chirurgia Vascolare 1998 December;5(4):237-46

Copyright © 2000 EDIZIONI MINERVA MEDICA

language: English, Italian

A comparative study of simultaneous determination by capillary zone electrophoresis and high performance liquid chromatography of allantoin, uric acid, hypoxanthine and xanthine in atherosclerotic plaque

Setacci C., Giubbolini M., Cappelli A., Palasciano G., Arezzini L., Landini T., Porcelli B., Terzuoli L.

From the * Cattedra di Chirurgia Vascolare Istituto di Biochimica e di Enzimologia Università degli Studi, Siena, Italy

Background. Allantoin, ­uric ­acid (UA), hypox­an­thine (Hx) and xan­thine (X) are ­enhanced dur­ing oxi­da­tive ­stress. We ­want deter­mine ­these metab­olites in ath­e­ros­cle­rot­ic ­plaque, in ­which oxi­da­tive ­stress is ­known, to ­improve our knowl­edge on ath­ero­scler­o­sis and to ­give a fur­ther ­help in the ­plaque char­ac­ter­iza­tion. In ­this prel­i­minary ­work, we pro­pose a com­par­a­tive ­study for the simul­ta­ne­ous deter­mi­na­tion of ­these com­pounds by cap­il­lary ­zone elec­tro­pho­re­sis (CE) and ­high per­for­mance liq­uid chrom­a­tog­ra­phy (­HPLC), to eval­u­ate the meth­od ­most suit­able for ­this anal­y­sis. This ­study is ­made ­both ­using stan­dards and carot­id ­plaque.
Methods. Surgical spec­i­mens ­were ­obtained ­from ­patients under­go­ing carot­id endar­te­rec­to­my. Electrophoretic sep­ar­a­tions ­were per­formed a Waters Quanta 4000 instru­ment; for ­HPLC sep­ar­a­tion a Varian Vista 5500.
Results. The ­HPLC meth­od ­enables res­o­lu­tion in gra­di­ent of the ­four ­compounds in 30 min, ­while CE meth­od in 20 min.
Conclusions. The CE and ­HPLC sep­ar­a­tions of allan­toin, UA, Hx and X may be ­used for a sat­is­fac­to­ry deter­mi­na­tion in ­vivo, in ­short ­time. Moreover the CE sep­ar­a­tion ­gave ­more pre­cise allan­toin dos­age, ­even ­when the con­cen­tra­tion is low.