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The Journal of Sports Medicine and Physical Fitness 2020 Apr 06

DOI: 10.23736/S0022-4707.20.10278-0

Copyright © 2020 EDIZIONI MINERVA MEDICA

language: English

LDH isoenzyme 5 is an index of early onset muscle soreness (EOMS) during prolonged running

Katsuyuki TOKINOYA 1, 2, Keisuke ISHIKURA 3, Yasuko YOSHIDA 4, 5, Song-Gyu RA 6, Takehito SUGASAWA 4, Atsushi AOYAGI 7, Yoshiharu NABEKURA 8, Kazuhiro TAKEKOSHI 4, Hajime OHMORI 8

1 Doctoral Program in Sports Medicine, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan; 2 Research Fellow of the Japan Society for the Promotion of Science, Tokyo, Japan; 3 Prefectural Education Center, Sojo University, Kumamoto, Japan; 4 Laboratory of Laboratory/Sports medicine, Division of Clinical Medicine, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan; 5 Department of Medical Technology, Faculty of Health Sciences, Tsukuba International University, Tsuchiura, Japan; 6 Faculty of Sports and Health Science, Fukuoka University, Fukuoka, Japan; 7 Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Japan; 8 Faculty of Health and Sport Sciences, University of Tsukuba, Tsukuba, Japan


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BACKGROUND: Muscle soreness is also induced during prolonged running such as a full marathon, and muscle soreness and increased damage markers are detected immediately after such a running. We named this muscle soreness, early onset muscle soreness (EOMS). Additionally, lactate dehydrogenase (LDH) level which has some isoenzyme is increased immediately after prolonged exercise. However, it is unclear that EOMS is related to muscle damage markers on prolonged running. This study aimed to determine at which point EOMS, and muscle damage markers are related to EOMS during prolonged running.
METHODS: We studied 11 male subjects who habitually perform aerobic exercise. They ran 30 km at 90% of ventilatory threshold intensity. Every 10 km, we estimated perceived muscle soreness, and sampled blood to measure muscle and liver damage, inflammation, and oxidative stress (d-ROM and BAP) markers.
RESULTS: Muscle soreness score lower limbs were significantly appeared at 20 km compared to that at 0 km. Serum lactate dehydrogenase (LDH) level increased at 30 km compared to that at 0 km. LDH isoenzymes 3, 4, and 5, and neutrophils significantly increased at 30 km compared to those at 0 km. Serum LDH isoenzyme 5 and change in aspartate aminotransferase significantly increased at 20 km. In addition, there was a significant correlation between the thigh NRS and amount of serum LDH isoenzyme 5 from 0 km to 20 km. d-ROM and BAP increased at 10 km compared to those at 0 km.
CONCLUSIONS: EOMS started to occur at 20 km during a 30 km running task. Our data suggest that LDH isoenzyme 5 is a marker of occurrence in EOMS during prolonged running.


KEY WORDS: Muscle damage markers; Marathon; 30km; Oxidative stress; Isoenzyme

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