Home > Journals > The Journal of Sports Medicine and Physical Fitness > Past Issues > The Journal of Sports Medicine and Physical Fitness 1999 December;39(4) > The Journal of Sports Medicine and Physical Fitness 1999 December;39(4):269-74

CURRENT ISSUE
 

JOURNAL TOOLS

eTOC
To subscribe PROMO
Submit an article
Recommend to your librarian
 

ARTICLE TOOLS

Reprints
Permissions

 

ORIGINAL ARTICLES   

The Journal of Sports Medicine and Physical Fitness 1999 December;39(4):269-74

Copyright © 2000 EDIZIONI MINERVA MEDICA

language: English

In vitro apoptosis of lymphocytes after exposure to levels of corticosterone observed following submaximal exercise

Hoffman-Goetz L., Zajchowski S.

Department of Health Studies and Gerontology, University of Waterloo, Waterloo, Ontario, Canada


PDF


Back­ground. To eval­uate the in ­vitro ­effects of cor­ti­cos­te­rone, equiv­a­lent to ­blood ­levels meas­ured ­after a ­single sub­max­imal tread­mill exer­cise, on apop­tosis and ­necrosis of ­mouse ­thymic and periph­eral lym­pho­cytes.
­Methods. Experi­mental ­design: anal­ysis of var­i­ance ­with inde­pen­dent fac­tors of ­time (0, 90, 210 min­utes of incu­ba­tion) and con­cen­tra­tion of cor­ti­cos­te­rone (0, 150, 250, 450, 850 ng/ml). Meas­ures: per­centage of apop­totic, ­necrotic, and ­viable thy­moc­ytes and sple­noc­ytes deter­mined by ­flow cytom­etry ­using ­Annexin V-­FITC anti­body and pro­pidium ­iodide.
­Results. ­There was a sig­nif­i­cantly ­higher % of apop­totic thy­moc­ytes at 210 min at the ­higher cor­ti­cos­te­rone con­cen­tra­tions but not in % of apop­totic sple­noc­ytes at the ­same ­time ­point. For ­both lym­phoid pop­u­la­tions, cor­ti­cos­te­rone incu­ba­tion was asso­ciated ­with a ­higher % of ­necrotic ­cells at 210 min­utes but not at 0 or 90 min­utes. For thy­moc­ytes, the inter­ac­tion (­time x con­cen­tra­tion) was sig­nif­i­cant, ­with ­greater % ­necrosis ­observed at the ­lower (150 and 250 ng/ml) con­cen­tra­tions of cor­ti­cos­te­rone.
Con­clu­sions. The ­data sug­gest ­that in ­vitro expo­sure to cor­ti­cos­te­rone, at phys­io­log­ical con­cen­tra­tions (≤450 ng/ml) ­observed ­after a mod­erate exer­cise ­stress, ­induces apop­tosis in thy­moc­ytes and ­necrosis in ­both thy­moc­ytes and sple­noc­ytes. The impli­ca­tions for exer­cise-medi­ated glu­co­cor­ti­coid reg­u­la­tion of ­immune func­tion ­remain to be inves­ti­gated.

top of page