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THE QUARTERLY JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING

A Journal on Nuclear Medicine and Molecular Imaging


A Journal on Nuclear Medicine and Molecular Imaging
Affiliated to the Society of Radiopharmaceutical Sciences and to the International Research Group of Immunoscintigraphy
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The Quarterly Journal of Nuclear Medicine and Molecular Imaging 2017 Aug 28

DOI: 10.23736/S1824-4785.17.02975-2

Copyright © 2017 EDIZIONI MINERVA MEDICA

language: English

Comparison of 99mTc-UBI 29-41, 99mTc-Ciprofloxacin, 99mTc-Ciprofloxacin dithiocarbamate and 111In-biotin for targeting experimental Staphylococcus aureus and Escherichia coli foreign-body infections: an ex-vivo study

Sveva AULETTA 1, Daniela BALDONI 2, Michela VARANI 1, Filippo GALLI 1, Iman A. HAJAR 3, Adriano DUATTI 3, Guillermina FERRO-FLORES 4, Andrej TRAMPUZ 5, Alberto SIGNORE 1

1 Nuclear Medicine, Department of Medical-Surgical Sciences and of Translational Medicine, Faculty of Medicine and Psychology, “Sapienza” University, Rome, Italy; 2 Infectious Diseases Research Laboratory, Department of Biomedicine, University Hospital, Basel, Switzerland; 3 Laboratory of Nuclear Medicine, Department of Radiological Sciences, University of Ferrara, Ferrara, Italy; 4 Departamento de Materiales Radioactivos, Instituto Nacionale de Investigaciones Nucleares, Gerencia de Aplicaciones Nucleares en Salud, Ocoyoacac, Estado de Mexico, Mexico; 5 Septic Surgery Unit, Center for Musculoskeletal Surgery, Charité, University of Medicine, Berlin, Germany


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BACKGROUNG: Diagnosis of implant-associated infection is challenging. Several radiopharmaceuticals have been described but direct comparisons are limited. Here we compared in vitro and in an animal model 99mTc-UBI, 99mTc-Ciprofloxacin, 99mTcN-CiproCS2 and 111In-DTPA-biotin for targeting E. coli (ATCC 25922) and S. aureus (ATCC 43335). METHODS: Stability controls were performed with the labelled radiopharmaceuticals during 6 h in saline and serum. The in vitro binding to viable or killed bacteria was evaluated at 37 °C and 4 °C. For in vivo studies, Teflon cages were subcutaneously implanted in mice, followed by percutaneous infection. Biodistribution of i.v. injected radiolabelled radiopharmaceuticals were evaluated during 24 h in cages and dissected tissues.
RESULTS: Labelling efficiency of all radiopharmaceuticals ranged between 94% and 98%, with high stability both in saline and in human serum. In vitro binding assays displayed a rapid but poor bacterial binding for all tested agents. Similar binding kinetic occurred also with heat-killed and ethanol-killed bacteria. In the tissue cage model, infection was detected at different time points: 99mTc-UBI and 99mTcN-CiproCS2 showed higher infected cage/sterile cage ratio at 24 h for both E. coli and S. aureus; 99mTc-Ciprofloxacin at 24 h for both E. coli and at 4 h for S. aureus; 111In-DTPA-biotin accumulates faster in both E. coli and S. aureus infected cages.
CONCLUSIONS: 99mTc-UBI, 99mTcN-CiproCS2 showed poor in vitro binding but good in vivo binding to E. coli only. 111In-DTPA-biotin showed poor in vitro binding but good in vivo binding to S. aureus and poor to E. coli. 99mTc-Ciprofloxacin showed poor in vitro binding but good in vivo binding to all tested bacteria. The mechanism of accumulation in infected sites remains to be elucidated.


KEY WORDS: Tissue cage model - 99mTc-UBI 29-41 - 99mTc-Ciprofloxacin - 111In-biotin - Infection imaging

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Publication History

Article first published online: August 28, 2017
Manuscript accepted: July 26, 2017
Manuscript revised: June 19, 2017
Manuscript received: January 23, 2017

Cite this article as

Auletta S, Baldoni D, Varani M, Galli F, Hajar IA, Duatti A, et al. Comparison of 99mTc-UBI 29-41, 99mTc-Ciprofloxacin, 99mTc-Ciprofloxacin dithiocarbamate and 111In-biotin for targeting experimental Staphylococcus aureus and Escherichia coli foreign-body infections: an ex-vivo study. Q J Nucl Med Mol Imaging 2017 Aug 28. DOI: 10.23736/S1824-4785.17.02975-2

Corresponding author e-mail

alberto.signore@uniroma1.it