Home > Journals > Journal of Neurosurgical Sciences > Past Issues > Articles online first > Journal of Neurosurgical Sciences 2020 Feb 04



Publishing options
To subscribe
Submit an article
Recommend to your librarian


Publication history
Cite this article as



Journal of Neurosurgical Sciences 2020 Feb 04

DOI: 10.23736/S0390-5616.19.04819-7


language: English

Induction of large cerebral aneurysms by intraperitoneal administration of β-aminopropionitrile fumarate in male rats

Yusuke SHIMODA, Naoki NAKAYAMA , Takuya MORIWAKI, Takeo ABUMIYA, Masahito KAWABORI, Kota KURISU, Masayuki GEKKA, Masaaki HOKARI, Yasuhiro ITO, Kiyohiro HOUKIN

Department of Neurosurgery, Hokkaido University Faculty of Medicine, Sapporo, Hokkaido, Japan


BACKGROUND: It is necessary and useful to obtain an experimental model which steadily and rapidly induces aneurysms for investigation of the pathogenesis of cerebral aneurysm. We attempted to examine whether intraperitoneal administration of β-aminopropionitrile fumarate (BAPN-F) with additional treatments of induced hypertension and hemodynamic stress could steadily and rapidly induce aneurysms in male rats.
METHODS: Seven-week-old male Sprague-Dawley rats pretreated with ligation of left common carotid and bilateral posterior renal arteries were administrated BAPN-F intraperitoneally. Induction rate and size of aneurysms was investigated with varying dose and duration of BAPN-F administration (low dose; 400 mg/kg/week for 4 or 8 weeks and high dose; 2800 mg/kg/week for 8 or 12 weeks).
RESULTS: Induction rate in the high-dose groups was significantly higher (p<0.01) than that in the low-dose groups. Making comparisons between 8 and 12 weeks of the high-dose groups, while there was no difference in induction rate (8 weeks; 85.2% vs 12 weeks; 76.9%), aneurysmal size was larger in 12 weeks (8 weeks; 127.5 μm, vs 12 weeks; 181.7 μm in terms of median) but lethal intrathoracic hemorrhage was increased in 12 weeks (8 weeks; 7.4% vs 12 weeks; 30.8%). Induction rate of large aneurysm was 22.2% and 30.8% in 8 and 12 weeks of the high-dose groups, respectively.
CONCLUSIONS: High-dose BAPN-F administration can cause high-frequency aneurysmal induction. Although there was the difference in size and mortality rate based on administration duration, intraperitoneal administration of 2800 mg/kg/week BAPN-F for 8 weeks would be suitable for aneurysmal induction.

KEY WORDS: Beta-aminopropionitrile fumarate; Intraperitoneal administration; Cerebral aneurysm; Rat model

top of page