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MINERVA STOMATOLOGICA

A Journal on Dentistry and Maxillofacial Surgery


Official Journal of the Italian Society of Odontostomatology and Maxillofacial Surgery
Indexed/Abstracted in: CAB, EMBASE, Index to Dental Literature, PubMed/MEDLINE, Scopus, Emerging Sources Citation Index


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Minerva Stomatologica 2018 Feb 09

DOI: 10.23736/S0026-4970.18.04118-3

Copyright © 2018 EDIZIONI MINERVA MEDICA

language: English

Vitamin D modulatory effect on cytokines expression by human gingival fibroblasts and periodontal ligament cells

Livia NASTRI 1 , Luigi GUIDA 1, Marco ANNUNZIATA 1, Nunzia RUGGIERO 2, Antonietta RIZZO 2

1 Multidisciplinary Department of Medical-Surgical and Odontostomatological Specialties, Unit of Periodontology and Implantology, University of Campania “Luigi Vanvitelli”, Naples, Italy; 2 Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology, University of Campania “Luigi Vanvitelli”, Naples, Italy


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BACKGROUND: It has been demonstrated that vitamin D exerts several functions other than those implied in the bone homeostasis. It has been published the vitamin D can act on many cells and tissues behaving also as a modulatory factor in the immune responses.
OBJECTIVE: The aim of our study was to evaluate the effect of active vitamin D3 (VD) on the expression of pro-inflammatory and anti-inflammatory cytokines (IL-6, IL-8, IL- 10 and IL-12) in human Gingival Fibroblasts (hGF) and human Periodontal Ligament cells (hPDLc) triggered by Porphyromonas gingivalis and Streptococcus pyogenes.
METHODS: Primary hGF and hPDLc pre-treated or not by VD (10-8 mol/l) were exposed to P. gingivalis and S. progenies for 24h. Production of IL-6, IL-8, IL-10 and IL-12 was evaluated by immunoenzymatic assay. mRNA of the same cytokines were evaluated by PCR.
RESULTS: IL-6 secretion increased by 25.2%(±2.1) up to 51%(±3.3) in VD treated hGF and hPDLc exposed to P. gingivalis and S. pyogenes, compared to VD not treated cells. IL-8 secretion decreased approximately by 30% in VD-treated hGF and hPDLc compared to VD not-treated cells. IL-12 secretion decreased by 60%. On the contrary, anti-inflammatory cytokine IL-10 increased by approximately 200%. mRNA PCR confirmed these results.
CONCLUSIONS: Within the limits of the study, the obtained results support the hypothesis of a modulatory role of VD on periodontal cells exposed to bacterial infection, reducing their inflammatory response and increasing the secretion of anti-inflammatory and modulatory cytokines. Consequently, it could be speculated that Vitamin D assessment, and its possible implementation in deficiency cases, could play a role in periodontal treatment.


KEY WORDS: Vitamin D - Gingival Fibroblasts - Cytokines - Inflammatory mediators - Human periodontal ligament cells

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Publication History

Article first published online: February 9, 2018
Manuscript accepted: February 1, 2018
Manuscript revised: January 29, 2018
Manuscript received: November 8, 2017

Cite this article as

Nastri L, Guida L, Annunziata M, Ruggiero N, Rizzo A. Vitamin D modulatory effect on cytokines expression by human gingival fibroblasts and periodontal ligament cells. Minerva Stomatol 2018 Feb 09. DOI: 10.23736/S0026-4970.18.04118-3

Corresponding author e-mail

livia.nastri@unicampania.it