Home > Journals > Minerva Stomatologica > Past Issues > Minerva Stomatologica 2012 October;61(10) > Minerva Stomatologica 2012 October;61(10):443-8





A Journal on Dentistry and Maxillofacial Surgery

Official Journal of the Italian Society of Odontostomatology and Maxillofacial Surgery
Indexed/Abstracted in: CAB, EMBASE, Index to Dental Literature, PubMed/MEDLINE, Scopus, Emerging Sources Citation Index




Minerva Stomatologica 2012 October;61(10):443-8


language: English

Introducing cut-points for salivary nitric oxide to distinguish periodontitis from the normal periodontium

Khorsavi Samani M. 1, 2, Poorsattar Bejeh Mir A. 2, 3, Kashiri M. 3, Gujeq D. 4

1 Department of Periodontology, Dentistry School, Babol University of Medical Sciences, Babol, Iran; 2 Dental Materials Research Center Babol University of Medical Sciences, Babol, Iran; 3 Dentistry Student Research Committee (DSRC), Dentistry School, Babol University of Medical Sciences, Babol, Iran; 4 Department of Biochemistry, Medical School, Babol University of Medical Sciences, Babol, Iran


AIM: We aimed to assess the diagnostic role of salivary nitric oxide (NO) to measure the periodontal health.
METHODS: In this case-control study, we included patients with mild to moderate chronic generalized periodontitis (cases) and compared them with the healthy individuals (controls). Bleeding index, plaque index, gingival index, clinical attachment loss and probing pocket depth were recorded. In addition, un-stimulated saliva was collected and nitric oxide content was quantified by spectrophotometer using Griess reaction.
RESULTS: Twenty-seven and 17 individuals were enrolled as the case and control groups, respectively. All clinical indices were worse in the control group as compared to the cases (P<0.05). Salivary nitric oxide in patients with periodontitis was 169 (±86) mg which was significantly higher than the individuals with healthy periodontium (63 [±17] mg, P<0.001). Using a receive operation characteristic (ROC) curve, cut-points of 45 and 110 were obtained with the respective negative predictive value and positive predictive value of 1 and 1.
CONCLUSION: A higher level of salivary nitric oxide was observed in patients with periodontitis in comparison to the healthy individuals. This may indicate that progression to the periodontitis is derived by NO-pathways. Based on our findings, a chair side detection of salivary nitric oxide may be a valuable screening tool, especially when subclinical periodontitis is suspected.

top of page

Publication History

Cite this article as

Corresponding author e-mail