Home > Journals > Minerva Stomatologica > Past Issues > Minerva Stomatologica 2010 May;59(5) > Minerva Stomatologica 2010 May;59(5):259-70

CURRENT ISSUE
 

JOURNAL TOOLS

eTOC
To subscribe
Submit an article
Recommend to your librarian
 

ARTICLE TOOLS

Reprints
Permissions

 

ORIGINAL ARTICLES   

Minerva Stomatologica 2010 May;59(5):259-70

Copyright © 2010 EDIZIONI MINERVA MEDICA

language: English, Italian

In vitro expression of osteoblastic phenotype on titanium surfaces

Ramaglia L. 1, Capece G. 1, Di Spigna G. 2, Esposito D. 1, Postiglione L. 2

1 Department of Dental and Maxillofacial Surgery Federico II University, Naples, Italy; 2 Department of Biology and Cellular and Molecular Pathology, Federico II University, Naples, Italy


PDF


AIM: Differentiation toward the osteoblastic phenotype is a complex phenomenon regulated by means of several factors. Numerous studies in vitro et in vivo showed that surface properties of titanium dental implants modulate cell proliferation and osteoblastic differentiation, affecting bone healing processes. Optimal superficial morphology is still controversial. The aim of the present study was to evaluate in vitro the effects of two different titanium surfaces on biological behaviour of human osteoblast-like cells SaOS-2 with regard to production of extra-cellular matrix (ECM) proteins.
MEHODS: Human osteoblast-like cells SaOS-2, cultured on commercially pure titanium disks with two different surface topographies, smooth and microstructured with sand-blasting and acid-etching treatment, were evaluated by investigating adhesion, proliferation and deposition of extra-cellular matrix (ECM) proteins Fibronectin, Tenascin and Collagen I.
RESULTS: The different values of cellular adhesion at three hours and the increase of SaOS-2 proliferation values at the different experimental times on both evaluated surfaces didn’t result statistically significant. ECM deposition analysis showed that Fibronectin, Tenascin and Collagen I were gradually produced, with not statistically significant differences for Fibrone-ctin and Tenascin and statistically significant differences for Collagen I.
CONCLUSION: Implant surface properties modulate in vitro the biological behavior of osteoblasts-like cells SaOS-2. SaOS-2 cells proliferate on both evaluated surfaces, showing a more organized differentiation towards an osteoblastic phenotype in ECM proteins production when cultured on microstructured surface.

top of page