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Minerva Stomatologica 2006 May;55(5):297-305


language: English, Italian

In vitro comparison of the cytotoxicity of two orthodontic composite resins

Nocca G. 1, Chimenti C. 2, Parziale V. 2, Gambarini G. 3, Giardina B. 1, 4, Lupi A. 4

1 Institute of Biochemistry and Clinical Biochemistry, Faculty of Medicine, Catholic University, Rome, Italy 2 Department of Surgical Sciences, School of Dentistry, University of L’Aquila, L’Aquila, Italy 3 Institute of Dentistry, Faculty of Medicine “La Sapienza” University, Rome, Italy 4 Institute of Chemistry of Molecular Recognition CNR, Section of Rome, Rome, Italy


Aim. Several studies have reported that dental resin-based materials release substances with biological activity: for this reason in this study we evaluated the in vitro cytopathic effects of a self-curing and a light-curing orthodontic composite resins by a cytotoxicity test.
Methods. The cytotoxic potential of specimens, prepared according to the manufacturer instructions, was evaluated using the thiazolyl blue tetrazolium bromide (MTT) assay on the mouse fibroblast cell line (3T3 Swiss) with 2 cells-material contact systems: the 24 h extracts method and the indirect toxicity method.
Results. The results obtained in this study elicit a close agreement between the 2 procedures; from the data obtained in the reported experimental conditions, it was possible to establish that the examined chemical-cured material is more cytotoxic than the light-cured one.
Conclusion. From a clinical point of view, the photo-polymerizable resins are undoubtedly more useful in the daily practice, because of the larger precision of the bonding obtainable by the greater period available for setting the brackets before their lock. The results obtained in this study, even considering the limits of the in vitro tests, represent a further favourable feature of the light-curing composite resins. However, further investigations about the influence of polymerization methods of the materials on the biological effects are suggested to contribute to the determination of the best clinical operative conditions.

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