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Minerva Pneumologica 2020 March;59(1):7-11

DOI: 10.23736/S0026-4954.20.01867-2

Copyright © 2020 EDIZIONI MINERVA MEDICA

language: English

The efficiency of the polymerase chain reaction methods for the detection of common pathogens in patients with chronic obstructive pulmonary disease

Hamidreza JAMAATI 1, Naghmeh BAHRAMI 2, 3, Payam TABARSI 4, Atefeh ABEDINI 5, Habib EMAMI 6, Mihan POURABDOLLAH 1, Mahya DAUSTANY 7, Yasaman HASANI 8, Hassan NOORBAZARGAN 9, Samaneh S. RASI VARAEI 10, Abdolreza MOHAMADNIA 1, 9

1 Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran; 2 Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran; 3 Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran; 4 Clinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran; 5 Tracheal Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran; 6 Tobacco Prevention and Control Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran; 7 Department of Biotechnology, Faculty of Sciences, Islamic Azad University, Tehran, Iran; 8 Private Practitioner, Teheran, Iran; 9 Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 10 Department of Clinical Biochemistry, Tehran University of Medical Sciences, Tehran, Iran



BACKGROUND: Chronic obstructive pulmonary disease (COPD) is a type of obstructive lung disease characterized by long-term breathing problems and poor airflow. It tends to worsen or progress over time. Some COPD cases may also develop bacterial infections, such as Haemophilus influenzae (Hi), Streptococcus pneumoniae (Sp), Mycoplasma pneumoniae (Mp), and Chlamydia pneumoniae (Cp). The present study aimed at employ the molecular methods conventional polymerase chain reaction (PCR) and real-time PCR as two complementary and more sensitive methods for detecting such infections.
METHODS: This is a descriptive cross-sectional study, for which a total of 109 samples of respiratory secretion were collected from the COPD patients suspected of having bacterial infection under the supervision of a pulmonologist. Then, the samples were subject to DNA extraction. The next step included some molecular experiments using available kits and specially designed primers. Finally, they were analyzed through statistical tests.
RESULTS: There was no significant difference in terms of gender (P value >0.05). The patients aged 62.4±12.2 on average. In the case of Mp, 3 out of 109 subjects were found to be positive using the kits, while two ones proved to be positive using the primers (the second method). In the case of Hi, 12 and 9 subjects were positive by using standard kits and employing second method, respectively. The obtained relative frequencies for Mp and Hi were respectively 3% and 11%.
CONCLUSIONS: Overall, the methods under study proved efficient in terms of sensitivity and accuracy as compared to both traditional/conventional methods and similar research findings. In addition, PCR with the designed primers exhibited acceptable results in terms of sensitivity and specificity as compared to PCR with commercial kits.


KEY WORDS: Pulmonary disease, chronic obstructive; Mycoplasma pneumoniae; Haemophilus Influenzae

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