ORIGINAL ARTICLE   

Minerva Pediatrics 2022 February;74(1):40-8

DOI: 10.23736/S2724-5276.17.04787-9

Copyright © 2017 EDIZIONI MINERVA MEDICA

language: English

Bcl-2 and caspase-9 serum levels in children and adolescents with idiopathic epilepsy and active seizures

Angeliki SKARDOUTSOU ¹, Panagiotis PRIMIKIRIS ¹, Charalampos TSENTIDIS ², Antonios MARMARINOS ², Dimitrios GOURGIOTIS ² ✉

¹ Second Department of Pediatrics, National and Kapodistrian University Medical School, P. and A. Kyriakou Children’s Hospital, Athens, Greece; ² Laboratory of Clinical Biochemistry and Molecular Diagnostics, Second Department of Pediatrics, National and Kapodistrian University Medical School, P. and A. Kyriakou Children’s Hospital, Athens, Greece

BACKGROUND: In the present study we investigated the levels of proapoptotic caspase-9 and antiapoptotic Bcl-2 proteins in the sera of children and adolescents with idiopathic epilepsy and tried to relate the findings to the patients’ clinical parameters.
METHODS: This retrospective study consisted of 118 children and adolescents with idiopathic epilepsy, categorized according to type and number of seizures, duration of the disease and the control of seizures and 30 age- and sex-matched controls. The relapse of seizures was taken into consideration.
RESULTS: Mean serum level between Bcl-2 and caspase-9 was significantly higher only in Bcl-2 patients, compared to controls (P≤0.0001) and (P=0.987) respectively. Significant difference in Bcl-2 level was found among the different types of focal seizures. Caspase-9 level was statistically different in patients with two or more seizures per month compared to those with one seizure per month (P=0.048). No correlation was found between Bcl-2 and caspase-9 levels and age, gender, seizure frequency, total number of seizures and the duration of epilepsy. No significant difference was found in patients with and without drug treatment.
CONCLUSIONS: Bcl-2 displays an association with apoptosis and highlights the potential of being a surrogate biomarker for active seizures and epilepsy. There is a significant difference in Bcl-2 serum level among the different types of focal seizures. Proapoptotic caspase-9 cannot act as a marker of active seizures and epilepsy. Caspase-9 serum level is increased acutely in controlled cases after a single relapse.

KEY WORDS: Epilepsy; Proto-oncogene proteins c-bcl-2; Caspase 9; Biomarkers; Child; Adolescent