Minerva Pediatrica 2018 Jan 29

DOI: 10.23736/S0026-4946.18.04991-5

Copyright © 2018 EDIZIONI MINERVA MEDICA

language: English

MW polyomavirus in diarrheal Italian infants

Massimiliano BERGALLO ¹ ✉, Ilaria GALLIANO ¹, Paola MONTANARI ¹, Marco RASSU ¹, Valentina DAPRÀ ¹, Chiara MERLINO ¹, Pier-Angelo TOVO ²

¹ Department of Public Health and Pediatric Sciences, University of Turin, Medical School, Turin, Italy; ² SC Pediatric U, Regina Margherita Hospital, Turin, Italy

BACKGROUND: MXPyV, like MWPyV, was identified in stool samples from children suffering diarrhea in Mexico. In this study, we used a home-made real time PCR to investigate the presence of this novel viruses in stool specimen collected from under-Five- Year-Old Children with gastroenteritis.
METHODS: A total of 192 fecal specimens previously screened for RV, ADV, NoV, HPeV and SaV, were tested for MWPyV with Taqman real time PCR.
RESULTS: The most commonly detected virus was NoV GII (33.8%), followed by RV (21.3%), SaV (10.9%), HPeV(8%), NoV GI (6.7%) and AdV (1%). Real time PCR detected MWPyV in 1/192 (0.5%) patients.
CONCLUSIONS: We detected MWPyV in 0.5% of fecal specimens collected from pediatric patients suffering gastroenteritis which is smaller than the previously reported in literature (4.4% in Australia and 12% Mexico).

KEY WORDS: MWPyV - Gastroenteritis - Pediatric - PCR