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Minerva Pediatrica 2017 May 04

DOI: 10.23736/S0026-4946.17.04717-X

Copyright © 2017 EDIZIONI MINERVA MEDICA

language: English

HERV-K and W expression in peripheral mononuclear cells of children with Henoch-Schönlein purpura and relation with TLRs activation

Massimiliano BERGALLO 1, Elisa LOIACONO 2, Ilaria GALLIANO 1, Paola MONTANARI 1, Licia PERUZZI 2, Pier-Angelo TOVO 1, Rosanna COPPO 2

1 Department of Public Health and Pediatric Sciences, University of Turin, Medical School, Turin, Italy; 2 Nephrology, Dialysis and Transplantation, University Hospital Città della Salute e della Scienza di Torino, Regina Margherita Hospital, Turin, Italy


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BACKGROUND: HERVs expression seems impaired in several diseases, ranging from autoimmune to neoplastic disorders. However, various stimuli may re-activate HERVs transcription. Henoch-Schönlein purpura is the most common cause of vasculitis in children. The role of microbial antigens in the pathogenesis of HSP remains elusive. TLRs are the first line of defense for the host to initiate an immune and inflammatory response. We aimed to investigate HERV, K and W expression in peripheral mononuclear cells of children with HSP and relation with TLRs activation.
METHODS: The study enrolled 63 children affected by HSP. We used RT-PCR to detect GAPDH in the peripheral blood mononuclear cells samples to normalize the data. Subsequently, the viral pol gene HERVs and TLRs transcripts in the same samples were assessed.
RESULTS: HERV K and W was expressed in all samples analyzed but the level of expression was much lower in the HSP that in HC p=0.0006 and p=0.0004 for HERV-K and –W respectively. TLR2 was iper-expressed in 39 vs 63 (62%) of the HSP, TLR3 in 23 vs 63 (42%), TLR4 in 42 vs 63 (66%) and TLR9 in 32 vs 63 (52%). The different expression were statistically significant only for TLR4 (p=0.0276) no for TLR2,3 and 9 (p=0.1251; 0.3964 and 0.1882 respectively). Spearman’s test demonstrated none correlation between the low expression of HERV-K and HERV-W and high expression of TLRs.
CONCLUSIONS: The results of the present study demonstrated that mRNA expression of HERV-K and –W and TLRs did not directly correlated.


KEY WORDS: HERV-K - HERV-W - TLRs - Henoch–Schönlein purpura

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