Minerva Medica 2020 Jun 12

DOI: 10.23736/S0026-4806.20.06566-0

Copyright © 2020 EDIZIONI MINERVA MEDICA

language: English

LINC00858 facilitates the malignant development of Wilms’ tumor by targeting miR-653-5p

Dan ZHOU ¹, Jilan WANG ², Suping XU ³ ✉, Zengming LI ⁴, Dan KOU ⁵

¹ Department of Nephrology, Shandong Shanxian Central Hospital, Heze, China; ² Department of Oncology Hematology, Rizhao Traditional Chinese Medicine Hospital, Rizhao, China; ³ Blood Purification Center, Weifang Second People's Hospital, Weifang, China; ⁴ Department of Health Management, PLA Rocket Force Characteristic Medical Center, Beijing, China; ⁵ Department of Economic Management, Medical Research Department, Pla Rocket Force Characteristic Medical Center, Beijing, China

BACKGROUND: To uncover the clinical significance of LINC00858 in the development of Wilms’ tumor and the potential molecular mechanism.
METHODS: LINC00858 levels in Wilms’ tumor species and cell lines were determined by quantitative real-time polymerase chain reaction (qRT-PCR). The clinical significance of LINC00858 in influencing pathological features and prognosis in patients with Wilms’ tumor was analyzed. Proliferative and migratory changes in Wilms’ tumor cells with LINC00858 knockdown were assessed. The downstream gene of LINC00858 was verified by luciferase assay, and its involvement in the development of Wilms’ tumor was further explored.
RESULTS: LINC00858 was highly expressed in Wilms’ tumor tissues and cell lines. High level of LINC00858 was correlated to high rate of lymphatic metastasis and poor prognosis in patients with Wilms’ tumor. Knockdown of LINC00858 suppressed proliferative and migratory potentials in HFWT and 17-94 cells. MiR-653-5p was targeted by LINC00858. It was lowly expressed in Wilms’ tumor tissues and negatively regulated by LINC00858. Knockdown of miR-653-5p partially abolished the regulatory effects of LINC00858 on proliferative and migratory potentials in Wilms’ tumor cells.
CONCLUSIONS: LINC00858 is highly expressed in Wilms’ tumor species, and correlated to lymphatic metastasis rate and overall survival in patients with Wilms’ tumor. Knockdown of LINC00858 suppresses Wilms’ tumor cells to proliferate and migrate via targeting miR-653-3p.

KEY WORDS: LINC00858; MiR-653-5p; Wilms’ tumor; Malignant development