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Minerva Medica 2020 Apr 07

DOI: 10.23736/S0026-4806.20.06565-9


language: English

MiR-30b-5p promotes myocardial cell apoptosis in rats with myocardial infarction through regulating Wnt/β-catenin signaling pathway

Feng CHI 1, Li FENG 2, Yuzhu LI 1, Shuoran ZHAO 1, Weiting YUAN 3, Yinsong JIANG 1, Lin CHENG 4

1 Department of Critical Care Medicine, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, China; 2 Department of Pharmacy, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, China; 3 Department of Emergency, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, China; 4 Department of Cardiovascular Medicine, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, China


BACKGROUND: To screen differentially expressed micro ribonucleic acids (miRNAs) in the heart tissues of rats with myocardial infarction (MI), to explore the relationship between miR-30b-p and MI, and to further research the action mechanism of miR-30b-p.
METHODS: 1) Rat MI models were established. Then 3 rats in experimental (MI) group and 3 rats in sham operation (Sham) group were selected, RNAs were extracted from heart tissues, and differentially expressed miRNAs in the two groups were screened using transcriptome sequencing techniques. 2) TargetScan was applied to predict miR-30b-5p target genes, which then were collected and subjected to enrichment analysis conducted using Metascape. 3) Myocardial cells were extracted from MI rats and transfected with miR-30b-5p inhibitor. Then the changes in cell proliferation and apoptosis were detected by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-diphenytetrazoliumromide (MTT) assay and flow cytometry, respectively. 4) The expressions of β-actin, β-catenin, Caspase-3 and cleaved Caspase-3 were detected in myocardial cells transfected with miR-30b-5p viaWestern blotting (WB).
RESULTS: 1) A total of 85 differentially expressed miRNAs were detected in the heart of MI rats, and miR-30b-5p expression was down-regulated (P<0.01). 2) In addition, target genes regulated by miR-30b-5p participated in the vascular development, miRNA metabolic process and Wnt signaling pathway. 3) In comparison with miR-negative control (NC) transfection, miR-30b-5p inhibitor transfection promoted the proliferation of myocardial cells but inhibited their apoptosis (P<0.05). 4) Compared with those in miR-NC transfection group, the expressions of β-catenin and cleaved Caspase-3 proteins were down-regulated in miR-30b-5p inhibitor transfection group (P<0.01).
CONCLUSIONS: MiR-30b-5p is involved in the proliferation and apoptosis of myocardial cells via regulating the Wnt/β-catenin signaling pathway, and it may become a potential target for the diagnosis of MI in clinic.

KEY WORDS: Myocardial infarction; MiR-30b-5p; Wnt; β-catenin; Target gene

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